Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) Antibody, CD45.2, 104

Pricing & Availability

Clone: 104 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-5.2, LCA
Isotype: Mouse (SJL) IgG2a, κ
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Product Citations: publications

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285018

50 µg

269€

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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: B10.S mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106283 (BioLegend Cat. No. 285018)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45.2 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD45.2	104	FC
FITC anti-mouse CD45.2	104	FC
PE anti-mouse CD45.2	104	FC
Purified anti-mouse CD45.2	104	FC,Block,IHC-F,IP
APC anti-mouse CD45.2	104	FC
Alexa Fluor® 488 anti-mouse CD45.2	104	FC
Alexa Fluor® 647 anti-mouse CD45.2	104	FC
Pacific Blue™ anti-mouse CD45.2	104	FC
Alexa Fluor® 700 anti-mouse CD45.2	104	FC
APC/Cyanine7 anti-mouse CD45.2	104	FC
PerCP anti-mouse CD45.2	104	FC
PerCP/Cyanine5.5 anti-mouse CD45.2	104	FC
PE/Cyanine7 anti-mouse CD45.2	104	FC
Brilliant Violet 421™ anti-mouse CD45.2	104	FC
Brilliant Violet 570™ anti-mouse CD45.2	104	FC
Brilliant Violet 650™ anti-mouse CD45.2	104	FC
Brilliant Violet 510™ anti-mouse CD45.2	104	FC
Brilliant Violet 785™ anti-mouse CD45.2	104	FC
Brilliant Violet 605™ anti-mouse CD45.2	104	FC
Purified anti-mouse CD45.2 (Maxpar® Ready)	104	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2	104	FC
Brilliant Violet 711™ anti-mouse CD45.2	104	FC
Alexa Fluor® 594 anti-mouse CD45.2	104	IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2	104	FC
TotalSeq™-A0157 anti-mouse CD45.2	104	PG
TotalSeq™-B0157 anti-mouse CD45.2	104	PG
TotalSeq™-C0157 anti-mouse CD45.2	104	PG
Brilliant Violet 750™ anti-mouse CD45.2	104	FC
Spark Blue™ 550 anti-mouse CD45.2	104	FC
Spark NIR™ 685 anti-mouse CD45.2	104	FC
Spark UV™ 387 anti-mouse CD45.2	104	FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™)	104	FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™)	104	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02.06.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.