- Clone
- E13-161.7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sca-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mouse pre-T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.
The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7 - Additional Product Notes
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For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
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Application References
(PubMed link indicates BioLegend citation) -
- Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
- Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
- van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
- van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
- Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
- Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
- Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
- Artinger EL, et al. 2013. PNAS. 110:12000. PubMed
- RRID
-
AB_3662477 (BioLegend Cat. No. 285137)
Antigen Details
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
-
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Function
- Regulates B and T cell responses
- Cell Type
- B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709. - Gene ID
- NA
- UniProt
- View information about Ly-6A/E on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
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We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
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Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
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We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
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Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
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Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All Ly-6A/E Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC,IHC-F,IHC-P,IP |
Biotin anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
FITC anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
PE anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
APC anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
Alexa Fluor® 647 anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
PE/Dazzle™ 594 anti-mouse Ly-6A/E (Sca-1) | E13-161.7 | FC |
Spark Red™ 718 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™) | E13-161.7 | FC |
Spark Blue™ 574 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™) | E13-161.7 | FC |
Spark Blue™ 550 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™) | E13-161.7 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Ly-6A/E (Sca-1)
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Biotin anti-mouse Ly-6A/E (Sca-1)
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FITC anti-mouse Ly-6A/E (Sca-1)
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PE anti-mouse Ly-6A/E (Sca-1)
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APC anti-mouse Ly-6A/E (Sca-1)
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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1)
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Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1)
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Alexa Fluor® 647 anti-mouse Ly-6A/E (Sca-1)
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Pacific Blue™ anti-mouse Ly-6A/E (Sca-1)
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PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1)
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APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1)
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PE/Dazzle™ 594 anti-mouse Ly-6A/E (Sca-1)
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Spark Red™ 718 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™)
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Spark Blue™ 550 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™)
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