- Clone
- SA045E1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-8RB, GRO/MGSA, Cmkar2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD182, also known as CXCR2, is a G-protein coupled receptor with seven transmembrane regions that is involved in chemotaxis, neutrophil activation, and angiogenesis. CXCR2 is expressed by neutrophils, basophils, a subset of T cells, monocytes, macrophages, natural killer (NK) and natural killer T cells (NKT). Its ligands are CXCL1, CXCL2, CXCL3, and CXCL5.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CXCR2-transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- RRID
-
AB_3106360 (BioLegend Cat. No. 285108)
Antigen Details
- Structure
- G-protein coupled receptor and seven transmembrane regions.
- Distribution
-
Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer (NK) cells.
- Function
- Chemotaxis, neutrophil activation, and angiogenesis.
- Ligand/Receptor
- CXCL1, CXCL2, CXCL3, and CXCL5.
- Cell Type
- Basophils, Macrophages, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Spaan AN, et al. 2014. Nat. Commun. 5:5438.
2. Natsuaki Y, et al. 2014. Nat. Immunol. 15:1064.
3. Hsieh CY, et al. 2014. J. Immunol. 193:3693.
4. Diana J and Lehuen A. 2014. EMBO Mol. Med. 6:1090.
5. Stoolman JS, et al. 2014. J. Immunol. 193:564. - Gene ID
- 12765 View all products for this Gene ID
- UniProt
- View information about CD182 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD182 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse CD182 (CXCR2) | SA045E1 | FC |
Purified anti-mouse CD182 (CXCR2) | SA045E1 | FC |
Alexa Fluor® 647 anti-mouse CD182 (CXCR2) | SA045E1 | FC |
PerCP/Cyanine5.5 anti-mouse CD182 (CXCR2) | SA045E1 | FC |
PE anti-mouse CD182 (CXCR2) | SA045E1 | FC |
Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2) | SA045E1 | FC |
PE/Dazzle™ 594 anti-mouse CD182 (CXCR2) | SA045E1 | FC |
PE/Cyanine7 anti-mouse CD182 (CXCR2) | SA045E1 | FC |
APC anti-mouse CD182 (CXCR2) | SA045E1 | FC |
Biotin anti-mouse CD182 (CXCR2) | SA045E1 | FC |
Spark Red™ 718 anti-mouse CD182 (CXCR2) (Flexi-Fluor™) | SA045E1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-mouse CD182 (CXCR2)
-
Purified anti-mouse CD182 (CXCR2)
-
Alexa Fluor® 647 anti-mouse CD182 (CXCR2)
-
PerCP/Cyanine5.5 anti-mouse CD182 (CXCR2)
-
PE anti-mouse CD182 (CXCR2)
-
Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2)
-
PE/Dazzle™ 594 anti-mouse CD182 (CXCR2)
-
PE/Cyanine7 anti-mouse CD182 (CXCR2)
-
APC anti-mouse CD182 (CXCR2)
-
Biotin anti-mouse CD182 (CXCR2)
-
Spark Red™ 718 anti-mouse CD182 (CXCR2) (Flexi-Fluor™)
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