Spark UV™ 387 anti-human CD16 Antibody, CD16, 3G8

Pricing & Availability

Clone: 3G8 (See other available formats)
Regulatory Status: RUO
Workshop: V NK80
Other Names: FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype: Mouse IgG1, κ
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Product Citations: publications

3G8_SparkUV387_CD16_Antibody_042123 — Human peripheral blood lymphocytes were stained with anti-human CD56 (NCAM) APC and anti-human CD16 (clone 3G8) Spark UV™ 387 (left) or stained with anti-human CD56 (NCAM) APC only (right).

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Size

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302077

25 tests

152€

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302078

100 tests

312€

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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human PMN cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm.

Excitation Laser

Ultraviolet Laser (355 nm)

Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation¹⁸. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶, immunoprecipitation³, stimulation of NK cell proliferation⁴, blocking of phagocytosis⁵, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
Hoshino S, et al. 1991. Blood 78:3232. (Stim)
Tamm A, et al. 1996. Immunol. 157:1576. (Block)
Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)

RRID

AB_2941462 (BioLegend Cat. No. 302077)
AB_2941462 (BioLegend Cat. No. 302078)

Antigen Details

Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: Aggregated IgG, IgG-antigen complex
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
Gene ID: 2214 View all products for this Gene ID
UniProt: View information about CD16 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Myeloid-Derived Suppressor Cells

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?: Yes

Other Formats

View All CD16 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD16	3G8	FC
Biotin anti-human CD16	3G8	FC
FITC anti-human CD16	3G8	FC
Brilliant Violet 711™ anti-human CD16	3G8	FC
PE anti-human CD16	3G8	FC
PE/Cyanine5 anti-human CD16	3G8	FC
Purified anti-human CD16	3G8	FC,CyTOF®,Block,IHC-F,IP,Stim
APC/Cyanine7 anti-human CD16	3G8	FC
PE/Cyanine7 anti-human CD16	3G8	FC
Alexa Fluor® 488 anti-human CD16	3G8	FC
Alexa Fluor® 647 anti-human CD16	3G8	FC
Pacific Blue™ anti-human CD16	3G8	FC
Alexa Fluor® 700 anti-human CD16	3G8	FC
PerCP/Cyanine5.5 anti-human CD16	3G8	FC
PerCP anti-human CD16	3G8	FC
Brilliant Violet 421™ anti-human CD16	3G8	FC
Brilliant Violet 570™ anti-human CD16	3G8	FC
Brilliant Violet 605™ anti-human CD16	3G8	FC
Brilliant Violet 650™ anti-human CD16	3G8	FC
Brilliant Violet 785™ anti-human CD16	3G8	FC
Brilliant Violet 510™ anti-human CD16	3G8	FC
Ultra-LEAF™ Purified anti-human CD16	3G8	FC,CyTOF®,Block,IHC-F,IP,Stim
Purified anti-human CD16 (Maxpar® Ready)	3G8	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD16	3G8	FC
APC/Fire™ 750 anti-human CD16	3G8	FC
PE anti-human CD16	3G8	FC
APC anti-human CD16	3G8	FC
Pacific Blue™ anti-human CD16	3G8	FC
PE/Dazzle™ 594 anti-human CD16	3G8	FC
TotalSeq™-A0083 anti-human CD16	3G8	PG
TotalSeq™-B0083 anti-human CD16	3G8	PG
TotalSeq™-C0083 anti-human CD16	3G8	PG
PE/Cyanine7 anti-human CD16	3G8	FC
PE/Fire™ 640 anti-human CD16	3G8	FC
FITC anti-human CD16	3G8	FC
Spark YG™ 581 anti-human CD16	3G8	FC
APC/Fire™ 750 anti-human CD16	3G8	FC
TotalSeq™-D0083 anti-human CD16	3G8	PG
APC/Fire™ 810 anti-human CD16	3G8	FC
GMP APC anti-human CD16	3G8	FC
GMP PE/Dazzle™ 594 anti-human CD16	3G8	FC
GMP PE anti-human CD16	3G8	FC
Spark Red™ 718 anti-human CD16	3G8	FC
GMP Pacific Blue™ anti-human CD16	3G8	FC
GMP FITC anti-human CD16	3G8	FC
Spark Blue™ 515 anti-human CD16	3G8	FC
Spark UV™ 387 anti-human CD16	3G8	FC
PerCP/Cyanine5.5 anti-human CD16	3G8	FC
GMP PE/Cyanine7 anti-human CD16	3G8	FC
GMP APC/Fire™ 750 anti-human CD16	3G8	FC
Brilliant Violet 750™ anti-human CD16	3G8	FC
Spark Blue™ 550 anti-human CD16	3G8	FC
GMP PerCP/Cyanine5.5 anti-human CD16	3G8	FC
Spark YG™ 593 anti-human CD16	3G8	FC
Spark NIR™ 685 anti-human CD16	3G8	FC
Spark Violet™ 500 anti-human CD16	3G8	FC
Spark Blue™ 574 anti-human CD16 (Flexi-Fluor™)	3G8	FC
Spark PLUS UV395™ anti-human CD16	3G8	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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