Spark YG™ 581 anti-human CD25 Antibody, CD25, M-A251

Pricing & Availability

Clone: M-A251 (See other available formats)
Regulatory Status: RUO
Workshop: IV A053
Other Names: IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype: Mouse IgG1, κ
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PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 FITC and anti-human CD25 (clone M-A251) Spark YG™ 581 (left) or CD4 FITC only (right).

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356153

25 tests

100€

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356154

100 tests

212€

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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4⁺ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PHA-induced lymphocyte cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections¹ and spatial biology (IBEX)^2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.
RRID: AB_2890784 (BioLegend Cat. No. 356153)
AB_2890784 (BioLegend Cat. No. 356154)

Antigen Details

Structure: Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution: Activated T and B cells, monocytes/macrophages, Tregs
Interaction: Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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PE anti-human CD25	M-A251	FC
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Alexa Fluor® 700 anti-human CD25	M-A251	FC
Brilliant Violet 510™ anti-human CD25	M-A251	FC
PE/Dazzle™ 594 anti-human CD25	M-A251	FC
Biotin anti-human CD25	M-A251	FC
Alexa Fluor® 647 anti-human CD25	M-A251	FC,SB
Pacific Blue™ anti-human CD25	M-A251	FC
PerCP anti-human CD25	M-A251	FC
APC/Fire™ 750 anti-human CD25	M-A251	FC
Brilliant Violet 711™ anti-human CD25	M-A251	FC
Brilliant Violet 785™ anti-human CD25	M-A251	FC
Brilliant Violet 605™ anti-human CD25	M-A251	FC
KIRAVIA Blue 520™ anti-human CD25	M-A251	FC
PE/Fire™ 700 anti-human CD25	M-A251	FC
APC/Fire™ 810 anti-human CD25	M-A251	FC
Spark NIR™ 685 anti-human CD25 Antibody	M-A251	FC
Spark YG™ 581 anti-human CD25	M-A251	FC
PE/Fire™ 640 anti-human CD25 Antibody	M-A251	FC
PE anti-human CD25	M-A251	FC
PerCP/Cyanine5.5 anti-human CD25	M-A251	FC
APC/Fire™ 750 anti-human CD25	M-A251	FC
PE/Cyanine7 anti-human CD25	M-A251	FC
APC anti-human CD25	M-A251	FC
PE/Cyanine5 anti-human CD25	M-A251	FC
FITC anti-human CD25	M-A251	FC
Spark Red™ 718 anti-human CD25	M-A251	FC
GMP PE anti-human CD25	M-A251	FC
GMP PE/Cyanine7 anti-human CD25	M-A251	FC
PerCP/Fire™ 780 anti-human CD25	M-A251	FC
PE/Fire™ 744 anti-human CD25	M-A251	FC
PerCP/Fire™ 806 anti-human CD25	M-A251	FC
Brilliant Violet 650™ anti-human CD25	M-A251	FC
Brilliant Violet 750™ anti-human CD25	M-A251	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 12.17.2020

For Research Use Only. Not for diagnostic or therapeutic use.

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