Ultra-LEAF™ Purified anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO expressed, recombinant mouse IL-12 p70

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA - Quality tested
Neut, ICFC, IP, WB - Reported in the literature, not verified in house

 

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection^1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard.
Neutralization^1,2,4,5,6,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for relevant formats of this clone) include: Western blotting³, immunoprecipitation³, and intracellular immunofluorescent staining and flow cytometric analysis.
Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)

1. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
2. Reichmann G, et al. 1999. J. Immunol. 163:3354.
3. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
4. Neucath M, et al. 1995. J. Exp. Med. 182:1281.
5. Cousens LP, et al. 1999 J. Exp. Med. 189:1315.
6. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
7. O'Connell PJ, et al. 2006. Blood 107:1010.
8. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
9. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
10. Sugimoto K, et al. 2007. Gastroenterology 133:124. PubMed

Product Citations

1. Luo WJ, et al. 2022. Elife. 11: . PubMed
2. Nomura A, et al. 2023. Life Sci Alliance. 6:. PubMed
3. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed

RRID

AB_11148948 (BioLegend Cat. No. 505307)
AB_11148948 (BioLegend Cat. No. 505308)
AB_11148948 (BioLegend Cat. No. 505309)
AB_11148948 (BioLegend Cat. No. 505310)
AB_11148948 (BioLegend Cat. No. 505311)
AB_11148948 (BioLegend Cat. No. 505312)

Antigen Details

Structure

Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)

Bioactivity

IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of T_H1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me

Cell Sources

Dendritic cells, monocytes/macrophages, B cells, T cells

Cell Targets

T cells, NK cells

Receptors

IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.

Regulation

Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23

Gene ID

16160 View all products for this Gene ID

UniProt

View information about IL-12/IL-23 p40 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Sandwich ELISA Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis