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- Clone
- W21057A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD154, TNF-related activator protein (TRAP), GP39, TNFSF5
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Biotinylated recombinant CD40 (TNFRSF5)-Fc chimera (Cat. No. 777214) (black circles) binds to immobilized recombinant human CD40L (TNFSF5) (Cat. No. 591706). Ultra-LEAF™ purified anti-human CD154 (CD40L) (clone W21057A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG1, κ isotype control (Cat. No. 400432) (gray triangles) does not have effect. This antibody blocks the binding of 0.3 µg/mL Biotinylated human CD40 (TNFRSF5)-Fc chimera to 1 µg/mL immobilized recombinant human CD40L (TNFSF5). ND50 range: 0.024 - 0.15 µg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 626653 | 100 µg | 344€ | ||||
| 626654 | 1 mg | 672€ | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD40L is a type II membrane protein that shares 78% amino acid identity with its mouse counterpart. Human CD40L is a polypeptide of 261 amino acids, consisting of 22, 24, and 215 amino acids for the cytoplasmic, transmembrane, and extracellular domains respectively. Soluble forms of CD40L (sCD40L) have been identified, and an 18 kD fragment has the ability to form homotrimers and bind to CD40 to induce biological signals. The 18 kD protein has full activity in B cell proliferation and differentiation assays. Activated platelets express CD40L on their surface and constitute the major source of sCD40L (>95% in blood). Platelet-derived CD40L regulates neutrophil recruitment and lung damage in sepsis, and it has been suggested that MMP9 regulates CD40L shedding in abdominal sepsis. Platelet-associated CD40L has been linked to progression of atherosclerotic disease, and elevated plasma levels of sCD40L in patients seems to be a reliable predictor of cardiovascular events, such as atherosclerotic plaque rupture and acute coronary syndromes. Also, platelet-associated CD40L expression is increased in idiopathic thrombocytopenic purpura patients, and it has been speculated that platelet-associated-CD40L drives the activation of autoreactive B lymphocytes in this disease. In addition, high plasma levels of sCD40L have been connected to HIV-Associated Neurocognitive Disorders (HAND).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human CD40L (TNFSF5)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.3 µg/mL recombinant biotinylated human CD40 (TNFRSF5)-Fc (Cat. No. 777214) to immobilized 1 µg/mL recombinant human CD40L (TNFSF5) (Cat. No. 591706). ND50 = 0.024 - 0.15 µg/mL.
- RRID
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AB_3083417 (BioLegend Cat. No. 626653)
AB_3083417 (BioLegend Cat. No. 626654)
Antigen Details
- Structure
- Homotrimer
- Distribution
-
T cells, basophils, mast cells, activated platelets
- Function
- CD40L induces proliferation, differentiation, and immunoglobulin class switching in B cells; also prevents apoptosis in B cell. CD40L is induced in T cells after in vitro activation with PHA, PMA plus ionomycin, anti-CD3, anti-CD2, and CD28 engagement in anti-CD3 activation. CD40L is also induced by thrombin in platelets.
- Interaction
- B cells, neutrophils, follicular dendritic cells, dendritic cells, macrophages, epithelial cells, epithelial carcinoma cells, endothelial cells, smooth muscle cells, fibroblasts, hepatic stellate cells, and glomerular mesangial cells
- Ligand/Receptor
- CD40
- Biology Area
- Cell Biology, Costimulatory Molecules
- Molecular Family
- Cytokines/Chemokines, Growth Factors, Immune Checkpoint Receptors
- Antigen References
-
- Spriggs MK, et al. 1992. J Exp Med. 176:1543-50.
- Mazzei GJ, et al. 1995. J Biol Chem. 270:7025-8.
- Delmas Y, et al. 2005. Med. Sci. (Paris). 21:825-31.
- Meabed MH, et al. 2007. Haematology. 12:301.
- Sprangue DL, et al. 2008. Blood. 111:5028-36.
- Davidson DC, et al. 2012. PLoS One. 7: e51793.
- Rahman M, et al. 2013. J. Thromb. Haemost 11:1385-98.
- Gene ID
- 959 View all products for this Gene ID
- UniProt
- View information about CD154 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD154 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD154 (CD40L) | W21057A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CD154 (CD40L)
Biotinylated recombinant CD40 (TNFRSF5)-Fc chimera (Cat. No....
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