- Clone
- CD28.2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V-CD28.05
- Other Names
- T44, Tp44
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, Costim, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Nunes J, et al. 1993. Biochem. J. 293:835.
- Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
- Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
- Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
- Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
- Rivollier A, et al. 2004. Blood 104:4029. (Costim)
- Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
- Sheng W, et al. 2007.Elsevier 580:6819. PubMed
- Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
- Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
- Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
- Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC)
- Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
- Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
- Product Citations
-
- RRID
-
AB_11148949 (BioLegend Cat. No. 302933)
AB_11148949 (BioLegend Cat. No. 302934)
AB_11148949 (BioLegend Cat. No. 302943)
AB_11148949 (BioLegend Cat. No. 302944)
AB_11148949 (BioLegend Cat. No. 302959)
AB_11148949 (BioLegend Cat. No. 302960)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
- Distribution
-
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD80, CD86
- Cell Type
- B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191. - Gene ID
- 940 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD28 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD28
Human peripheral blood lymphocytes stained with APC CD28.2 -
Biotin anti-human CD28
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 FITC -
PE anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE -
PE/Cyanine5 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE/Cy... -
Purified anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE -
Alexa Fluor® 488 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 Alexa... -
Alexa Fluor® 700 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 Alexa... -
PerCP/Cyanine5.5 anti-human CD28
Human peripheral blood lymphocytes were stained with CD3 APC... -
Pacific Blue™ anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 Pacif... -
PE/Cyanine7 anti-human CD28
Human peripheral blood lymphocytes stained with CD28.2 PE/Cy... -
Ultra-LEAF™ Purified anti-human CD28
Human peripheral blood lymphocytes stained with LEAF™ purifi... Human peripheral blood mononuclear cells were stained with C... Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human CD28
Human peripheral blood lymphocytes were stained with CD28 (c... -
Brilliant Violet 510™ anti-human CD28
Human peripheral blood lymphocytes were stained with CD28 (c... -
Purified anti-human CD28 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 160Gd-an... -
PE/Dazzle™ 594 anti-human CD28
Human peripheral blood lymphocytes were stained with CD28 (c... -
Brilliant Violet 785™ anti-human CD28
Human peripheral blood lymphocytes were stained with CD28 (c... -
Brilliant Violet 650™ anti-human CD28
Human peripheral blood Lymphocytes were stained with CD28 (c... -
Brilliant Violet 711™ anti-human CD28
Human peripheral blood Lymphocytes were stained with CD28 (c... -
APC/Fire™ 750 anti-human CD28
Human peripheral blood lymphocytes were stained with CD28 (c... -
Alexa Fluor® 647 anti-human CD28
Human peripheral blood lymphocytes were stained with CD28 (c... -
TotalSeq™-A0386 anti-human CD28
-
TotalSeq™-B0386 anti-human CD28
-
TotalSeq™-C0386 anti-human CD28
-
Brilliant Violet 605™ anti-human CD28
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC/Cyanine7 anti-human CD28
Human peripheral blood lymphocytes were stained with True-St... -
PE anti-human CD28
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD28
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 750™ anti-human CD28
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Fire™ 810 anti-human CD28
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD28
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-D0386 anti-human CD28
-
Spark Violet™ 423 anti-human CD28
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP Ultra-LEAF™ Purified anti-human CD28 SF
Human peripheral blood lymphocytes were stained with GMP Ult... PBMC-derived T cells were activated in the presence of GMP U... -
GMP APC anti-human CD28
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD28
Typical results from human peripheral blood lymphocytes stai... -
GMP Ultra-LEAF™ Biotin anti-human CD28 SF
Human peripheral lymphocytes were stained with GMP Ultra-LEA... PBMC-derived T cells were activated in the presence of GMP U... -
PerCP/Cyanine5.5 anti-human CD28
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 515 anti-human CD28
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD28
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP/Cyanine5.5 anti-human CD28
Typical results from human peripheral blood lymphocytes stai...
Follow Us