- Clone
- W21071E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glucocorticoid-induced TNFR-related protein (GITR), CD357, AITR, Energen
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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624753 | 100 µg | 332€ | ||||
624754 | 1 mg | 956€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
NFRSF18 is a type I transmembrane protein that was initially identified from dexamethasone-treated murine T cell hybridoma cells, and was designated GITR (for glucocorticoid-induced TNFR family-related gene). The human TNFRSF18 has 56% amino acid sequence identity to the mouse receptor. The human and mouse receptors contain three cysteine rich domains (CRD) in their extracellular region. TNFRSF18 expression is up-regulated in human peripheral mononuclear cells after stimulation with anti-CD3/CD28 monoclonal antibodies. TNFRSF18 interacts with TRAF1 (TNF receptor-associated factor 1), TRAF2, and TRAF3, and induces nuclear factor NFkB activation via TRAF2. TNFRSF18 is a key player in the differentiation of thymic Tregs (tTregs), and expansion of both tTregs and peripheral Tregs (pTregs). Human regulatory T cells (Tregs) constitutively expresses TNFRSF18. TNFRSF18 and its ligand are involved in the pathogenesis of autoimmune disease. Soluble forms for TNFRSF18 and TNFSF18 were detected in the serum of patients with primary Sjögren's syndrome, and those levels correlated with disease severity. Other autoimmune and allergic diseases have been associated to TNFRSF18 /TNFSF18 interaction as demonstrated in disease models of gastritis, thyroiditis, experimental autoimmune encephalomyelitis, and allergic airway inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD357 (GITR)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.15 µg/mL human TNFSF18 (GITRL) to 2 µg/mL immobilized recombinant human GITR (TNFRSF18) Fc Chimera. ND50 range: 0.3-2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3083414 (BioLegend Cat. No. 624753)
AB_3083414 (BioLegend Cat. No. 624754)
Antigen Details
- Structure
- Homodimer
- Distribution
-
Immature and mature dendritic cells, B cells, macrophages, keratinocytes, endothelial cells, and retinal epithelial cells
- Function
- Differentiation of thymic Tregs (tTregs), and expansion of both tTregs and peripheral Tregs (pTregs). TNFRSF18 is stimulated in anti-CD3/CD28 activated T cells
- Interaction
- Macrophages, B cells, dendritic cells (DCs), and endothelial cells
- Ligand/Receptor
- TNFSF18 (AITRL, GITRL, TL6)
- Cell Type
- Lymphocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Nocentini G, et al. 1997. Proc. Natl. Acad. Sci. USA. 94:6216-21.
- Gurney AL, et al. 1999. Curr Biol. 9:215-8.
- Shimizu J, et al. 2002. Nat Immunol. 3:135-42.
- Tone M, et al. 2003. Proc Natl Acad Sci USA. 100:15059.
- Gan X, et al. 2013. Clin Dev Immunol. 2013:340751.
- Nocentini G, et al. 2012. Br J Pharmacol. 165:2089-99.
- Ronchetti S, et al. 2015. J Immunol Res. 2015:171520.
- Gene ID
- 8784 View all products for this Gene ID
- UniProt
- View information about CD357 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD357 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-human CD357 (GITR) | W21071E | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CD357 (GITR)
Recombinant Human TNFSF18 (GITRL) (Cat. No. 559204) (black c...
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