- Clone
- AER-37 (CRA-1) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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334647 | 100 µg | 216€ | ||||
334648 | 1 mg | 542€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Activ - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4
-
Application References
(PubMed link indicates BioLegend citation) -
- Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
- Suzukawa M, et al. 2005. Int. Immunol. 17:1249.
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
- RRID
-
AB_2819963 (BioLegend Cat. No. 334647)
AB_2819963 (BioLegend Cat. No. 334648)
Antigen Details
- Structure
- Ig superfamily, 50 kD
- Distribution
-
Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients
- Function
- Bind IgE, trigger IgE-mediated allergic response
- Ligand/Receptor
- IgE
- Cell Type
- Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
- Biology Area
- Immunology
- Molecular Family
- Fc Receptors
- Antigen References
-
1. Riske F, et al. 1991. J. Biol. Chem. 266:11245
2. Gounni AS, et al. 2001. FASEB J. 15:940.
3. Maurer D, et al. 1996. J. Immunol. 157:607
4. Maurer d, et al. 1994. J. Exp. Med. 179:745
5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92. - Gene ID
- 2205 View all products for this Gene ID
- UniProt
- View information about FcepsilonRIalpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All FcεRIα Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human FcεRIα
Human peripheral blood lymphocytes stained with purified AER... -
Biotin anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
FITC anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6... -
PE anti-human FcεRIα
Human peripheral blood leukocytes stained with AER-37 (CRA-1... -
Alexa Fluor® 647 anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6... -
PerCP anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6... -
APC anti-human FcεRIα
Human peripheral blood lymphocytes stained with CD203c (NP4D... -
Pacific Blue™ anti-human FcεRIα
Human peripheral leukocytes stained with CD203c (NP4D6) PE a... -
PE/Cyanine7 anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6... -
PerCP/Cyanine5.5 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Brilliant Violet 421™ anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Brilliant Violet 510™ anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Brilliant Violet 605™ anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
APC/Cyanine7 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Alexa Fluor® 700 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
PE/Dazzle™ 594 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Brilliant Violet 711™ anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Alexa Fluor® 488 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
TotalSeq™-A0352 anti-human FcεRIα
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TotalSeq™-C0352 anti-human FcεRIα
-
APC/Fire™ 750 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c ... -
Ultra-LEAF™ Purified anti-human FcεRIα
Human peripheral blood lymphocytes stained with purified AER... -
TotalSeq™-B0352 anti-human FcεRIα
-
TotalSeq™-D0352 anti-human FcεRIα
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