Ultra-LEAF™ Purified anti-human TSLPR Antibody

Pricing & Availability
Clone
W20296I (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20296I_ULEAF_TSLPR_Neut_091123
Recombinant human TSLP (Cat. No. 582402) (black circles) induces the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells. Ultra-LEAF™ purified anti-human TSLPR (clone W20296I) (purple squares) neutralizes the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells induced by recombinant human TSLP at 10 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.6-4 µg/mL.
  • W20296I_ULEAF_TSLPR_Neut_091123
    Recombinant human TSLP (Cat. No. 582402) (black circles) induces the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells. Ultra-LEAF™ purified anti-human TSLPR (clone W20296I) (purple squares) neutralizes the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells induced by recombinant human TSLP at 10 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.6-4 µg/mL.
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622353 100 µg 344€
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622354 1 mg 988€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. TSLP-R contains a WSXWS motif required for proper protein folding and a box 1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight of approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed on T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in an allergic response.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human TSLPR
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells induced by recombinant human TSLP at 10 ng/mL. ND50 range: 0.6 - 4 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3083411 (BioLegend Cat. No. 622353)
AB_3083411 (BioLegend Cat. No. 622354)

Antigen Details

Structure
Type I membrane receptor
Distribution

Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells; also expressed in heart, skeletal, muscle, and kidney cells.

Function
TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. It has also been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells
Interaction
Forms functional heterodimeric complex with IL-7R to bind TSLP
Ligand/Receptor
TSLP/TSLPR
Cell Type
Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Reche PA, et al. 2001. J Immunol. 167:336-43. 
  2. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23-5.
  3. Zhang W, et al. 2001. Biochem Biophys Res Commun. 281:878-83.
  4. Wang YH, et al. 2006. Immunity, 24:-838.
Gene ID
64109 View all products for this Gene ID
UniProt
View information about TSLPR on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09.11.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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