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- Clone
- A23078B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD30 antigen, CD30, CD30KI-1, CD30L receptor, cytokine receptor CD30, D1S166EKi-1, Ki-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant mouse CD30L (Cat. No. 553602) (black circles) binds to immobilized recombinant mouse CD30/TNFRSF8 protein. Ultra-LEAF™ purified anti-mouse Tnfrsf8 (CD30) (clone A23078B) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ Isotype Control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.8 µg/mL recombinant Mouse CD30L to 1 µg/mL immobilized recombinant mouse CD30/TNFRSF8. ND50 range: 0.1 - 0.8 µg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 667353 | 100 µg | 256€ | ||||
| 667354 | 1 mg | 636€ | ||||
CD30, also known as TNFRSF8, is a member of the tumor necrosis factor (TNF) receptor superfamily and was originally identified as a cell surface antigen Ki-1, on Hodgkin and Reed-Sternberg cells. CD30 can be cleaved by cell membrane-anchored metalloproteinases and then released from the cell as a soluble form protein. CD30 is expressed on activated T, B, and NK cells, but not on resting T, and B cells. CD30 is also highly expressed in, among others, classical Hodgkin lymphoma (HL) and anaplastic large cell lymphoma (ALCL). CD30 interacts with a number of proteins including TRAF1, TRAF2, TRAF3, TRAF5, NPM-ALK, TRAF-interacting protein, and CD30 ligand (CD153). Signaling through CD30 is thought to limit the proliferative potential of autoreactive CD8 effector T cells and protect against autoimmunity. CD30 mediates the signal transduction that leads to the activation of the nuclear factor kappaB (NF-kB) transcription factor. Coexpression of CD30 with TRAF1 augments NF-kB activation. In addition, CD30 also has been identified as a critical therapeutic target for lymphoma immunotherapy. CD30L/CD30 signaling has been implicated in the pathogenesis of several autoimmune and inflammatory conditions, such as rheumatoid arthritis (RA). Furthermore, CD30L/CD30 signaling appears to be involved in Th1 and Th2 cell responses and plays a key role in Th17 differentiation in mice.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Mouse CD30/TNFRSF8
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.8 µg/mL mouse CD30L (Cat. No. 553602) to immobilized 1 µg/mL recombinant mouse CD30/TNFRSF8. ND50 range: 0.1 - 0.8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Monomer
- Distribution
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Activated T, B cells, Th1, Th2, Th0, CD8 T cells, NK cells
- Function
- CD30 could induce survival, proliferation, differentiation, or apoptosis. CD30 regulates B cell growth and differentiation. CD30 expression in activated T cells depends on CD28 signal transduction or IL-4. CD30 signals augment T cell proliferation, thymocyte survival.
- Interaction
- CD4 T cells, Th1, Th2, mast cells, macrophages, dendritic cells, B cells, neutrophils
- Ligand/Receptor
- CD30L (CD153)
- Cell Type
- B cells, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Rossi FM, et al. 2001. FEBS Lett. 508:418-22.
- Trovato M, et al. 2001. Thyroid. 11:621-8.
- Ekstrom ES, et al. 2001. Placenta. 22:372-9.
- Tang C, et al. 2008. J Immunol. 181:6316-27.
- Sun X, et al. 2008. Gastroenterology. 134:447-58.
- Oflazoglu E, et al. 2009. Adv Exp Med Biol. 647:174-85.
- Gene ID
- 21941 View all products for this Gene ID
- UniProt
- View information about CD30 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD30 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD30 | A23078B | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD30
Recombinant mouse CD30L (Cat. No. 553602) (black circles) bi...
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