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Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD279 (PD-1) PE/Cyanine7 or CD366 (TIM3) PE (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right). -
Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD25 PerCP/Cyanine5.5 or CD69 Alexa Fluor® 700 (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right). -
Veri-Cells™ Activated (surface) PBMC stained with CD3 APC/Fire™ 750, CD4 FITC, and CD134 (OX40) APC or TIGIT (VSTM3) PE/Dazzle™ 594 (left). Plots were generated on CD3+ cells and gated based on corresponding isotype control stains (right). -
Veri-Cells™ Activated (surface) PBMC scatter profile (left) and stain for CD3 APC/Fire™ 750 (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 426701 | 10 tests | 90€ | ||||
| 426703 | 50 tests | 372€ | ||||
The reconstituted lyophilized human Activated (surface) PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as activated cell marker controls with minimal variability across experiments. These cells have been verified to work with commonly tested cell surface, as well as T cell activation markers such as CD3, CD4, CD8, CD25, CD366(TIM3), TIGIT, CD69, CD279 and CD134.
This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus using FDA approved methods. Biological tests are not 100% accurate. Use standard precautions when handling, and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.
Kit Contents
- Kit Contents
-
Cat. No. 426701
- 2 vials of Veri-Cell™ Activated (surface) PBMC (≥1 X 106 cells per vial)
- 1 vial of 0.7 ml Veri-Cells™ Buffer A Plus
Cat. No. 426703
- 10 vials of Veri-Cell™ Activated (surface) PBMC (≥1 X 106 cells/vial)
- 5 vials of 0.7 ml Veri-Cells™ Buffer A Plus
Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.
Product Details
- Formulation
- Cat. No. 426701: 2 vials each containing ≥1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).Cat. No. 426703: 10 vials each containing ≥1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
- Preparation
- The Veri-Cells™ PBMC Kit is prepared from 2 lyophilized human Activated (surface) PBMC and a vial of Veri-Cells™ Buffer A Plus. Veri-Cells Activated (surface) PBMC contain lyophilized PBMCs that had been stimulated using Concanavalin A (ConA) for 72 hours.
- Storage & Handling
- Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. Contact BioLegend Technical Support for information on the current lots
- Application
-
FC - Quality tested
- Recommended Usage
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50 µl per test (after reconstitution with 325 µl of Veri-Cells™ Buffer A Plus)
Once reconstituted, let the cells sit for 15 minutes at room temperature prior to staining.
Veri-Cells™ Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer. - Application Notes
-
The Veri-Cells™ Activated (surface) PBMC Kit can be used as a positive control for detecting T cell activation markers such as CD25, CD69, CD134, CD279, CD366 (TIM3) and TIGIT (VSTM3). Assessing the suitability of using this product as a control for end user’s molecule of interest is recommended, as each lot is verified based on the expression levels of markers mentioned above. For best results, Cell Staining Buffer is recommended for any washing steps. Product performance specifications are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.
Antibodies Used for Product Validation
Target
Clone
Fluorophore
CD3
SK7
APC/Fire™ 750
CD4
OKT4
PE/Cyanine7
CD25
M-A251
PerCP/Cyanine5.5
CD366/TIM3
F38-2E2
PE
TIGIT
A15153G
PE/Dazzle™ 594
CD69
FN50
Alexa Fluor® 700
CD279
A17188
FITC
CD134
Ber-ACT35
APC
Cell subset
Acceptance Criteria, % positive of lymphocyte gate
CD3+CD25+
>10%
CD3+CD69+
>5%
CD3+ CD134 (OX40)+
>5%
CD3+ CD366 (Tim-3)+
>1%
CD3+TIGIT (VSTM3)+
>10%
CD3+CD279 (PD-1)+
>8%
Note on indicated ranges: Percent positive values are based on gates set on cell stained with corresponding isotype for each marker. The antibodies used for testing were the following: CD3 APC/Cyanine7 (clone SK7), CD25 PerCP/Cyanine5.5 (clone M-A251), CD134 (OX40) APC (clone Ber-ACT35), CD69 Alexa Fluorâ 700 (clone FN50), CD279 (PD-1) FITC (clone A17188), CD366 (Tim-3) PE clone (F38-2E2), TIGIT (VSTM3) PE/Dazzle 594™ (clone A15153G). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.
Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Activated (Surface) PBMC. - Additional Product Notes
-
Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
Related FAQs
- Is washing required after staining?
-
Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.
- Can the cells be activated after rehydration?
- No, they cannot be activated.
- Are these cells virus/pathogen free?
- These are tested to be free of HIV, HBV, syphilis, and HCV.
- Are there any RBCs in Veri-cells™?
- There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.
- Are these cells viable?
- No they are not.
- Can a constant percentage of each population be expected in each lot?
- Yes, within each lot, cell populations remain constant.
- Are the cells fixed?
-
They are treated with our proprietary mixture prior to lyophilization.
- Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?
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For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.
- How do we QC these cells?
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Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range. For more information regarding the exact markers used for each product, please contact technical service.
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