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APC anti-human IL-2 Antibody

Pricing & Availability
Clone
MQ1-17H12 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
MQ1-17H12_APC_022508
PMA + ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with MQ1-17H12 APC and CD3 (UCHT1) PE
  • MQ1-17H12_APC_022508
    PMA + ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with MQ1-17H12 APC and CD3 (UCHT1) PE
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Cat # Size Price Quantity Check Availability Save
500309 25 tests 91€
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500311 50 µg 133€
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500310 100 tests 228€
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed recombinant human IL-2
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.

Clone MQ1-17H12 cross-reacts to Cat15

Application References

(PubMed link indicates BioLegend citation)
  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
  8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
  9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  13. Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
  14. Wu Z, et al. 2015. J Virol. 89:6435. PubMed
  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
Product Citations
  1. Keeton R 2023. Cell Reports Medicine. 4(1):100898. PubMed
  2. Freimer JW, et al. 2022. Nat Genet. 54:1133. PubMed
  3. Yang C, et al. 2023. Front Immunol. 14:1112672. PubMed
  4. Velnati S, et al. 2023. Front Immunol. 14:1043603. PubMed
  5. Li J, et al. 2023. Elife. :12. PubMed
  6. Colleton B, et al. 2009. J Virol. 83:6288. PubMed
  7. Hall VG, et al. 2022. Nat Immunol. 23:380. PubMed
  8. Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed
  9. Zhou X, et al. 2021. J Clin Transl Hepatol. 9:283. PubMed
  10. D, et al. 2016. Tuberculosis. 95: 470-475. PubMed
  11. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  12. Priyanto H, et al. 2021. J Clin Tuberc Other Mycobact Dis. 22:100214. PubMed
  13. Heitmann JS, et al. 2021. Nature. Online ahead of print. PubMed
  14. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  15. Qiu XM, et al. 2020. Reproduction. 251:159. PubMed
  16. Cohen KW, et al. 2021. Cell Reports Medicine. 2(7):100354. PubMed
  17. Keeton R, et al. 2021. Cell Host Microbe. 29:1611. PubMed
  18. Fuery A, et al. 2015. PLoS One. 10: 0133126. PubMed
  19. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  20. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  21. Cassioli C, et al. 2021. Front Cell Dev Biol. 9:673446. PubMed
  22. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  23. Ferreira VH, et al. 2022. Nat Commun. 13:4489. PubMed
  24. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  25. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
  26. Rochman Y, et al. 2015. PLoS One. 10:122198. PubMed
  27. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
RRID
AB_315096 (BioLegend Cat. No. 500309)
AB_315096 (BioLegend Cat. No. 500311)
AB_315096 (BioLegend Cat. No. 500310)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org
Go To Top Version: 3    Revision Date: 05.23.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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