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FITC anti-human CD33 Antibody

Pricing & Availability
Clone
HIM3-4 (See other available formats)
Regulatory Status
RUO
Workshop
V MA112
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HIM3-4
Human peripheral blood monocytes stained with HIM3-4 FITC
  • HIM3-4
    Human peripheral blood monocytes stained with HIM3-4 FITC
See FITC spectral data
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303303 25 tests 68€
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303304 100 tests 153€
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications include: immunofluorescence2.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations
  1. Liao J, et al. 2023. Nat Commun. 14:207. PubMed
  2. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  3. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  4. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  5. Gu H, et al. 2021. Immun Inflamm Dis. 9:1686. PubMed
  6. Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed
  7. Tamassia N, et al. 2008. Int Immunol. 1.204861111. PubMed
  8. Seman BG, et al. 2021. J Cell Sci. 134:. PubMed
  9. Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed
  10. Mori A, et al. 2021. Cells. :10. PubMed
  11. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  12. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  13. Lecciso M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01918. PubMed
  14. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  15. Shaw BC, et al. 2021. CRISPR J. 4:699. PubMed
  16. Schwarz F, et al. 2016. Proc Natl Acad Sci U S A. 113: 74 - 79. PubMed
RRID
AB_314343 (BioLegend Cat. No. 303303)
AB_314343 (BioLegend Cat. No. 303304)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells

Function
Adhesion and lectin activity
Ligand/Receptor
Sugar chains containing sialic acid
Cell Type
Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org
Go To Top Version: 2    Revision Date: 09.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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