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PerCP/Cyanine5.5 anti-human CD7 Antibody

Pricing & Availability
Clone
CD7-6B7 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-164
Other Names
gp40
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
CD7-6B7_PerCPCy55_CD7_Antibody_121516
Human peripheral blood lymphocytes were stained with CD7 (clone CD7-6B7) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • CD7-6B7_PerCPCy55_CD7_Antibody_121516
    Human peripheral blood lymphocytes were stained with CD7 (clone CD7-6B7) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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343115 25 tests 141€
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343116 100 tests 306€
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Description

CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG1a cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported (for the relevant formats) applications include proteogenomics2.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University Press.
  2. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
RRID
AB_2632911 (BioLegend Cat. No. 343115)
AB_2632911 (BioLegend Cat. No. 343116)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 40 kD
Distribution

T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets

Function
T cell activation
Ligand/Receptor
Cytoplasmic domain associates with P13-kinase
Cell Type
Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Stillwell R, et al. 2001. Immunol. Res. 24:31.
3. Rabinowich H, et al. 1994. J. Immunol. 152:517.

Gene ID
924 View all products for this Gene ID
UniProt
View information about CD7 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05.24.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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