- Clone
- W16197A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ACTB, Actin, cytoplasmic 1, Beta-actin
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
664801 | 25 µg | 81€ | ||||
664802 | 100 µg | 203€ |
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Zebrafish, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human ACTB peptide (1-13 a.a.).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Reactivity to Drosophila was only verified with the purified format.
- Product Citations
-
- RRID
-
AB_2721350 (BioLegend Cat. No. 664801)
AB_2721350 (BioLegend Cat. No. 664802)
Antigen Details
- Structure
- 375 amino acids with a predicted molecular weight of 42 kD.
- Distribution
-
Cytoplasm
- Function
- Actins are highly conserved proteins that are involved in cell motility.
- Interaction
- Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687. - Gene ID
- 60 View all products for this Gene ID
- UniProt
- View information about beta-actin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All β-actin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-β-actin | W16197A | WB |
Direct-Blot™ HRP anti-β-actin | W16197A | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-β-actin
Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain a... Total lysates (15 µg protein) from HeLa was resolved by elec... Western blot of purified anti-β-actin antibody (clone W16197... -
Direct-Blot™ HRP anti-β-actin
Whole cell lysates (15 µg protein) from HeLa was resolved by...
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