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Purified anti-Cytokeratin (pan reactive) Antibody

Pricing & Availability
Clone
C-11 (See other available formats)
Regulatory Status
RUO
Other Names
Cytokeratin, Keratin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C-11_cytokeratin_panreactive_antibody_1_031919
Whole cell extracts (15 µg protein) from Jurkat (negative control) and HeLa cells were resolved on a 4-20% Tris-glycine gel, transferred to a nitrocellulose membrane and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Cytokeratin (pan reactive) Antibody, clone C-11, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • C-11_cytokeratin_panreactive_antibody_1_031919
    Whole cell extracts (15 µg protein) from Jurkat (negative control) and HeLa cells were resolved on a 4-20% Tris-glycine gel, transferred to a nitrocellulose membrane and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-Cytokeratin (pan reactive) Antibody, clone C-11, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • C-11_cytokeratin_panreactive_antibody_2_080619
    MCF-7 cells were stained with purified anti- Cytokeratin (pan reactive) (C-11) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Nuclei were stained with DAPI (blue).
  • d.C-11_101807
    Hela cells stained with purified mouse monoclonal antibody against pan Cytokeratin (clone C-11), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
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628601 25 µg 81€
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628602 100 µg 175€
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Description

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Keratin enriched fraction from human epidermoid carcinoma.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
IP, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
  2. Bartek J, et al. 1991. J. Pathol. 164:215.
  3. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
  4. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  5. Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
  6. Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yamashita S, et al. 2018. Oncol Lett. 15:400. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Greier MDC, et al. 2023. Front Oncol. 13:1145817. PubMed
  4. Fang WB, et al. 2023. Front Oncol. 13:1130911. PubMed
  5. Nwosu ZC, et al. 2023. Nature. 618:151. PubMed
  6. Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed
  7. Florian S, et al. 2019. Proc Natl Acad Sci U S A. 116:11444. PubMed
  8. Kesavan R, et al. 2021. Int J Mol Sci. 22:. PubMed
  9. Yao Y, et al. 2017. Cytometry B Clin Cytom. 92:12:00. PubMed
  10. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  11. Baines KJ, et al. 2020. J Immunol. 204:694. PubMed
  12. Chernyavsky A, et al. 2007. J Biol Chem . 2.042361111. PubMed
  13. Bade RM, et al. 2021. Molecular Oncology. . PubMed
  14. Mayer J, et al. 2011. Cancer Genet. 204:589. PubMed
  15. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  16. Mukhtar E, et al. 2020. Cancer Drug Resist. 0.566666667. PubMed
RRID
AB_439774 (BioLegend Cat. No. 628601)
AB_439774 (BioLegend Cat. No. 628602)

Antigen Details

Structure
Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution

Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin pan reactive on UniProt.org
Go To Top Version: 7    Revision Date: 12.17.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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