Purified anti-mouse CD2 Antibody anti-CD2

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Clone: RM2-5 (See other available formats)
Regulatory Status: RUO
Other Names: LFA-2, T11, Ly-37, SRBC-R
Isotype: Rat IgG2b, λ
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RM2-5_Purified_021306 — C57BL/6 mouse splenocytes stained with purified RM2-5

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100102

500 µg

187€

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Description

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP, Block, Costim - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation¹, blocking of T cell A.I.C.D.², immunoprecipitation³, and co-induction of thymocyte maturation⁴. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).
RRID: AB_312649 (BioLegend Cat. No. 100102)

Antigen Details

Structure: Ig superfamily, 45-58 kD
Distribution: T cells, thymocytes, NK cells, B cells
Function: T cell activation, adhesion
Ligand/Receptor: CD48, lck
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
Gene ID: 12481 View all products for this Gene ID
UniProt: View information about CD2 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD2	RM2-5	FC
FITC anti-mouse CD2	RM2-5	FC
PE anti-mouse CD2	RM2-5	FC
Purified anti-mouse CD2	RM2-5	FC,IP,Block,Costim
APC anti-mouse CD2	RM2-5	FC
PE/Cyanine7 anti-mouse CD2	RM2-5	FC
PerCP/Cyanine5.5 anti-mouse CD2	RM2-5	FC
TotalSeq™-A0892 anti-mouse CD2	RM2-5	PG
Ultra-LEAF™ Purified anti-mouse CD2	RM2-5	FC,IP,Block,Costim
TotalSeq™-C0892 anti-mouse CD2	RM2-5	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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