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Alexa Fluor® 488 anti-human IgG Fc Antibody

Pricing & Availability
Clone
HP6017 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
HP6017_AF488_IgG_Antibody_FC_041714
Human DLL4-transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
  • HP6017_AF488_IgG_Antibody_FC_041714
    Human DLL4-transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
See Alexa Fluor® 488 spectral data
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This product has been discontinued, please use alternate products: Cat. Nos: 410705 and 410706 and Cat. No: 366921.

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Description

IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cross-Reactivity: Goat, Rabbit, Sheep, Horse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human immunoglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HP6017 reacts with IgG (Fc fragment) of all subclasses. This antibody does not react with IgA, IgM, purified light chains, or the Fab fragment of IgG. This clone is not recommended for detection of transmembrane IgG on B cells. We recommend using clone M1310G05 as an alternative.

Application References

(PubMed link indicates BioLegend citation)
  1. Reime CB, et al. 1984. Hybridoma 3:263.
  2. Jefferis R, et al. 1985. Immunol. Lett. 10:223.
Product Citations
  1. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  2. Morgan SB, et al. 2018. Front Immunol. 9:865. PubMed

Antigen Details

Structure
Homodimer formed by the constant region of the IgG heavy chain
Ligand/Receptor
CD16, CD32, CD64
Cell Type
B cells, Basophils, Dendritic cells, Macrophages, Mast cells, Neutrophils, NK cells, Platelets
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Innate Immunity
Molecular Family
Fc Receptors
Antigen References

1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.

Gene ID
3500 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgG Fc
Specificity Alt (DOES NOT SHOW ON TDS):
IgG Fc
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about IgG Fc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/06/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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