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APC anti-mouse CD19 Antibody

Pricing & Availability
Clone
1D3/CD19 (See other available formats)
Regulatory Status
RUO
Other Names
B4, CVID3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1D3slashCD19_APC_CD19_Antibody_092616
C57BL/6 mouse splenocytes were stained with CD19 APC (clone 1D3/CD19, filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • 1D3slashCD19_APC_CD19_Antibody_092616
    C57BL/6 mouse splenocytes were stained with CD19 APC (clone 1D3/CD19, filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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152409 25 µg £65
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152410 100 µg £191
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfected k562 cells expressing the extracellular domain of murine CD19.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
  2. Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
Product Citations
  1. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  2. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  3. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  4. Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed
  5. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  6. Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed
  7. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  8. Ma X, et al. 2023. Nat Commun. 14:1606. PubMed
  9. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  10. Prystaz K, et al. 2018. Am J Pathol. 188:474. PubMed
  11. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  12. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  13. Dignum T, et al. 2021. Cell Rep. 36:109675. PubMed
  14. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  15. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  16. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  17. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  18. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  19. Frazier T, et al. 2018. Adipocyte. 0.41875. PubMed
  20. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  21. Bonglack EN, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  22. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  23. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  24. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  25. Li H, et al. 2019. Front Immunol. 2.205555556. PubMed
  26. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  27. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  28. Nordin F, et al. 2020. BMC Pregnancy and Childbirth. 20(1):399. PubMed
  29. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  30. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  31. Thürmer M, et al. 2022. Nat Commun. 13:2982. PubMed
  32. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  33. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  34. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  35. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  36. Chonwerawong M, et al. 2020. Gastroenterology. 159:169. PubMed
  37. Spath S, et al. 2022. iScience. 25:104998. PubMed
RRID
AB_2629838 (BioLegend Cat. No. 152409)
AB_2629838 (BioLegend Cat. No. 152410)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells.

Function
Modulates B cell activation and differentiation.
Ligand/Receptor
CD21, CD81, Leu-13.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD19
Specificity Alt (DOES NOT SHOW ON TDS):
CD19
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 1    Revision Date: 09/26/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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