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APC anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
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Product Citations
publications
RMM-1_APC_042909.jpg
C57BL/6 splenocytes stained with RMM-1 APC
  • RMM-1_APC_042909.jpg
    C57BL/6 splenocytes stained with RMM-1 APC
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406509 100 µg £203
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Ridenour D, et al. 2014. Development. 141:1095. PubMed
  2. Leung G, et al. 2021. JCI Insight. 6:. PubMed
  3. Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed
  4. Ryszawy D, et al. 2014. Carcinogenesis. 35:1920. PubMed
  5. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  6. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  7. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  8. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
  9. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  10. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  11. Shukla V, et al. 2020. Molecular Cell. 77(2):384-394.e4.. PubMed
  12. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  13. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  14. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  15. Nick H, et al. 2012. Blood. 119:1370. PubMed
  16. Shibad V, et al. 2021. Front Immunol. 12:722451. PubMed
  17. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  18. Hoyt T, et al. 2015. Infect Immun . 83:743. PubMed
  19. Iype J, et al. 2019. J Immunol. 202:1417. PubMed
  20. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  21. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  22. Hirata T, et al. 2018. Nat Commun. 9:405. PubMed
  23. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  24. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  25. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  26. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
  27. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  28. Pan J, et al. 2022. Front Immunol. 13:812924. PubMed
  29. Mao C, et al. 2020. Antib Ther. 0.234027778. PubMed
  30. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  31. Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
  32. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_315059 (BioLegend Cat. No. 406509)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgM
Specificity Alt (DOES NOT SHOW ON TDS):
IgM
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about IgM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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