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Brilliant Violet 750™ anti-human CD357 (GITR) Antibody

Pricing & Availability
Clone
108-17 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF18, Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
108-17_BV750_CD357_Antibody_022720.png
Human peripheral blood lymphocytes were activated for three days with PHA, and then stained with CD3 PE only (left) or human peripheral blood lymphocytes were activated for three days with PHA, and then stained with CD3 PE and CD357 (clone 108-17) Brilliant Violet 750™ (right). Gates were established based on CD3 PE single colored stain.
  • 108-17_BV750_CD357_Antibody_022720.png
    Human peripheral blood lymphocytes were activated for three days with PHA, and then stained with CD3 PE only (left) or human peripheral blood lymphocytes were activated for three days with PHA, and then stained with CD3 PE and CD357 (clone 108-17) Brilliant Violet 750™ (right). Gates were established based on CD3 PE single colored stain.
Compare all formats See Brilliant Violet 750™ spectral data
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371229 25 tests £133
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371230 100 tests £291
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Description

GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human GITR-Fc chimera
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
RRID
AB_2876698 (BioLegend Cat. No. 371229)
AB_2876698 (BioLegend Cat. No. 371230)

Antigen Details

Structure
TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD.
Distribution

Activated lymphocytes.

Function
Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway.
Ligand/Receptor
GITRL
Cell Type
Lymphocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. van der Werf N, et al. 2011. J. Immunol. 187:1411.
2. Shimizu J, et al. 2002. Nat. Immunol. 3:135.
3. McHugh RS, et al. 2002. Immunity 16:311.
4. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.

Gene ID
8784 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD357
Specificity Alt (DOES NOT SHOW ON TDS):
CD357 (GITR)
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD357 on UniProt.org
Go To Top Version: 1    Revision Date: 02/27/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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