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GoInVivo™ Purified anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-14 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279, PD 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMP1-14_GoInVivo_CD279_Antibody_051217
Anti-mouse PD-1 inhibits the binding of mouse PD-1 to PD-L1. Immobilized PD-L1-Fc chimeric protein was incubated with PD-1 in the presence of anti-mouse PD-1 (clone RMP1-14, purple squares) or isotype control (clone RTK2758, purple circles). Bound PD-1 was detected with an HRP conjugate system. Clone RMP1-14 inhibits PD-L1/PD-1 interaction in a dose dependent manner.
  • RMP1-14_GoInVivo_CD279_Antibody_051217
    Anti-mouse PD-1 inhibits the binding of mouse PD-1 to PD-L1. Immobilized PD-L1-Fc chimeric protein was incubated with PD-1 in the presence of anti-mouse PD-1 (clone RMP1-14, purple squares) or isotype control (clone RTK2758, purple circles). Bound PD-1 was detected with an HRP conjugate system. Clone RMP1-14 inhibits PD-L1/PD-1 interaction in a dose dependent manner.
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114114 5 mg £527
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114115 25 mg £1618
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114116 50 mg £2446
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114111 100 mg £3650
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114112 500 mg £12603
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114113 1 g £19563
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC, WB, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
  2. Personal Communication (WB)
  3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
  4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
  5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
  7. Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)
Product Citations
  1. Zhu Y, et al. 2023. Sci Immunol. eade4656:8. PubMed
  2. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  3. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  4. Sakamoto K, et al. 2022. Sci Rep. 12:8121. PubMed
  5. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  6. Panagioti E, et al. 2021. J Clin Invest. 131:. PubMed
  7. Bok I, et al. 2022. JID Innov. 2:100076. PubMed
RRID
AB_2566089 (BioLegend Cat. No. 114114)
AB_2566089 (BioLegend Cat. No. 114115)
AB_2566089 (BioLegend Cat. No. 114116)
AB_2566089 (BioLegend Cat. No. 114111)
AB_2566089 (BioLegend Cat. No. 114112)
AB_2566089 (BioLegend Cat. No. 114113)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD279
Specificity Alt (DOES NOT SHOW ON TDS):
CD279
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC,WB,FA
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 5    Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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