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ELISA MAX™ Deluxe Set Human CXCL5

Pricing & Availability
Regulatory Status
RUO
Other Names
Epithelial-derived neutrophil-activating protein 78 (ENA-78), GCP-2, AMCF-II, small inducible cytokine B5, (SCY-B5)
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Product Citations
publications
CXCL5_Human_ELISA_Kit_Deluxe_122013
  • CXCL5_Human_ELISA_Kit_Deluxe_122013
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440904 5 plates £238
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.


CXCL5 is a member of the CXC family of chemokines, also known as epithelial activated peptide 78 (ENA-78). The precursor of CXCL5 contains 114 amino acids, with a signal peptide of 36 amino acids which is cleaved to generate the 78 amino acids mature protein (8 kD). Human mature CXCL5 shares 57% amino acid sequence identity with mouse and rat CXCL5. Human CXCL5 also shares 41 - 77% sequence identity with CXCL1, CXCL2, CXCL3, CXCL4 CXCL6, CXCL7 and CXCL8.

CXCL5 is expressed by monocytes/macrophages, endothelial cells, vascular smooth muscle cells, epithilial cells, and eosinophils. CXCL5 is an inflammatory cytokine which binds to the cell surface receptor CXCR2 and induces chemotaxis of neutrophils to a target site. High levels of CXCL5 have been associated with prostate, gastric, and pancreatic cancer progression.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human CXCL5 (ENA-78) ELISA MAX™ Capture Antibody (200X)
  • Human CXCL5 (ENA-78) ELISA MAX™ Detection Antibody (200X)
  • Human CXCL5 (ENA-78) Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Deng J, et al. 2021. JCI Insight. 6:146133. PubMed
Sensitivity
0.5 pg/mL
Standard Range
2.0-125 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
6374 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CXCL5
Specificity Alt (DOES NOT SHOW ON TDS):
CXCL5
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA
UniProt
View information about CXCL5 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 09/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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