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MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit

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A single cell suspension from collagenase digested 4T1-induced tumors were depleted of CD45+ cells using CD45 Nanobeads (Cat. No. 480028). CD326+ (Ep-CAM) cells were then isolated using MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD. Left plot: CD45-CD326 (Ep-CAM)+ cells after isolation. Right plot: Cells before isolation.
  • MojoSort_Mouse_CD326_Selection_Kit_1_092619.png
    A single cell suspension from collagenase digested 4T1-induced tumors were depleted of CD45+ cells using CD45 Nanobeads (Cat. No. 480028). CD326+ (Ep-CAM) cells were then isolated using MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD. Left plot: CD45-CD326 (Ep-CAM)+ cells after isolation. Right plot: Cells before isolation.
  • MojoSort_Mouse_CD326_Selection_Kit_2_092619.png
    A single cell suspension mixture of 90% C57BL/6 mouse splenocyte with 10% 4T1 mouse cell line was prepared for positive selection of CD326+ (Ep-CAM) cells using the MojoSort™ Mouse CD326 (Ep-CAM) selection Kit. Left plot: CD326+ (Ep-CAM) cells after isolation. Right plot: Cells before isolation. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD.
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480141 20 tests £150
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480142 100 tests £295
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Description

Mouse CD326 (Ep-CAM) cells are selected by incubating the sample with biotin conjugated anti-mouse CD326 antibody after blocking with TruStain FcX (anti-mouse CD16/32 antibody) then followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD326 (Ep-CAM) expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. In addition to CD326 (Ep-CAM) expression on epithelial and tumor cells, CD326 is also expressed on subtypes of leukocytes, which should be depleted using MojoSort™ Mouse CD45 Nanobeads before CD326 positive selection.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480141:

  • 1 vial containing 200 µL of Biotin anti-mouse CD326 antibody (clone G8.8)
  • 1 vial containing 200 µL of Streptavidin Nanobeads
  • 1 vial containing 200 µL of each TruStain FcX (Anti-mouse CD16/32 antibody)


For Cat# 480142:

  • 1 vial containing 1mL of each of Biotin anti-mouse CD326 antibody (clone G8.8)
  • 1 vial containing 1mL of each of Streptavidin Nanobeads
  • 1 vial containing 1mL of each of TruStain FcX (Anti-mouse CD16/32 antibody)

Product Details

Verified Reactivity
Mouse
Formulation
Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2 with stabilizer.

Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

5 µL of TruStain FcX (Anti-mouse CD16/32 antibody) for 1 x 107 cells in 100 µL of buffer.

10 µL of Biotin anti-mouse CD326 antibody for 1 x 107 cells in 100 µL of buffer.

10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for the positive selection of mouse CD326 (Ep-CAM) cells from single-cell preparations of epithelial or tumor cells.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

To find out the dilution to use this product in separation columns please download the CoA.

Antibody or cocktail dilution to use in column: 1.6X
SAV Particle dilution to use in columns: 16X

Antigen Details

Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
CD326
Specificity Alt (DOES NOT SHOW ON TDS):
CD326
App Abbreviation (DOES NOT SHOW ON TDS):
Cell Sep - Pos
UniProt
View information about CD326 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 05/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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