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PE anti-human CD111 (Nectin-1) Antibody

Pricing & Availability
Clone
R1.302 (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus Receptor Related 1 Protein (PRR1), Nectin-1, HveC
Isotype
Mouse IgG1, κ
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Product Citations
publications
R1dot302_PE_051409.jpg
Human erythroleukemia cell line TF-1 stained with R1.302 PE
  • R1dot302_PE_051409.jpg
    Human erythroleukemia cell line TF-1 stained with R1.302 PE
See PE spectral data
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340404 100 tests £247
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Description

CD111, known as poliovirus receptor related 1 protein (PRR1), Nectin-1 and HveC, is a 75 kD type I transmembrane glycoprotein. A member of the nectin family and Ig gene superfamily, CD111 is broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells. CD111 functions as one of the herpes simplex virus 1 (HSV-1) and HSV-2 receptors and is an adhesion molecule involved in the formation of cell junctions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Nectin-1 transfected NIH/3T3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation, Western blot, immunohistochemical staining of frozen tissue sections, block HSV-1 and HSV-2 entry into cells

Application References

(PubMed link indicates BioLegend citation)
  1. Cocchi F, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:15700.
  2. Martinez WM and Spear PG. 2002. J. Virol. 76:7255.
  3. Reymond N,et al. 2004. J. Exp Med. 199:1331.
  4. Gillory LA, et al. 2013. PLoS One. 8:77753. PubMed
Product Citations
  1. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  2. Wang CQ, et al. 2023. Cells. 12: . PubMed
  3. Friedman G, et al. 2016. Neuro Oncology. 18: 227 - 235. PubMed
  4. Friedman GK, et al. 2018. Sci Rep. 8:13930. PubMed
  5. Gillory L, et al. 2013. PLoS One. 8:77753. PubMed
  6. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
RRID
AB_2174152 (BioLegend Cat. No. 340404)

Antigen Details

Structure
75 kD type I transmembrane glycoprotein, Nectin family, Ig superfamily
Distribution

Myelomonocytic cells, megakaryocytes, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells.

Function
Adhesion, receptor for Herpes simplex Virus 1 (HSV-1) and HSV-2
Ligand/Receptor
Nectin-1, -2, -3, and -4, CD155, HSV-1 and HSV-2
Cell Type
Megakaryocytes, Hematopoietic stem and progenitors, Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola h, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication.
2. Simpson SA, et al. 2005. J. Neuroviral. 11:208.
3. Akhtar J, et al. 2008. Ophthalmol. Vis. Sci. 49:4026.

Gene ID
5818 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD111
Specificity Alt (DOES NOT SHOW ON TDS):
CD111
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD111 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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