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PE anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
F38-2E2 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_F38-2E2_PE_020810
Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom).
  • A_F38-2E2_PE_020810
    Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom).
  • B_F38-2E2_PE_020810
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345005 25 tests £105
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345006 100 tests £247
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Tim-3 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
  2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
  3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations
  1. Fang L, et al. 2013. Neuro Oncology. 15:1479. PubMed
  2. Zhang T, et al. 2022. Am J Reprod Immunol. 87:e13524. PubMed
  3. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  4. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  5. Ceppi F, et al. 2022. Cancer Immunol Res. 10:856. PubMed
  6. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  7. Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed
  8. Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed
  9. Jung IY, et al. 2022. Sci Transl Med. 14:eabn7336. PubMed
  10. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  11. Poonia B, Pauza C 2014. PLoS One. 9:88884. PubMed
  12. Yang H, et al. 2019. Mol Metab. 23:24. PubMed
  13. Ho T, et al. 2016. Blood. 128: 1671 - 1678. PubMed
  14. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  15. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  16. Wei F, et al. 2013. Proc Natl Acad Sci U S A. 110:2480. PubMed
  17. Wang S, et al. 2021. Exp Ther Med. 21:37. PubMed
  18. Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed
  19. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  20. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  21. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  22. Andersch L, et al. 2019. BMC Cancer. 19:895. PubMed
  23. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  24. Zhang Y, et al. 2012. J Leukoc Biol. 91:189. PubMed
  25. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  26. Ho JY, et al. 2021. Mol Ther Methods Clin Dev. 21:237. PubMed
  27. Bhattacharya P, et al. 2020. Elife. 9:00. PubMed
  28. Fromentin R, et al. 2016. PLoS Pathog. 12: 1005761. PubMed
  29. Jin C, et al. 2016. EMBO Mol Med. 8: 702 - 711. PubMed
  30. Barresi V, et al. 2020. J Clin Med. 9:00. PubMed
  31. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  32. Gorris MAJ, et al. 2018. J Immunol. 200:347. PubMed
  33. Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
  34. Finney OC, et al. 2019. J Clin Invest. 130:. PubMed
RRID
AB_2116576 (BioLegend Cat. No. 345005)
AB_2116576 (BioLegend Cat. No. 345006)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9
Cell Type
Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.

Gene ID
84868 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD366
Specificity Alt (DOES NOT SHOW ON TDS):
CD366
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD366 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD366 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD366 (Tim-3) F38-2E2 FC
PE anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 421™ anti-human CD366 (Tim-3) F38-2E2 FC
Ultra-LEAF™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC anti-human CD366 (Tim-3) F38-2E2 FC
PE/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
PerCP/Cyanine5.5 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 605™ anti-human CD366 (Tim-3) F38-2E2 FC
FITC anti-human CD366 (Tim-3) F38-2E2 FC
Purified anti-human CD366 (Tim-3) (Maxpar® Ready) F38-2E2 FC,CyTOF®
Brilliant Violet 711™ anti-human CD366 (Tim-3) F38-2E2 FC
APC/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 785™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 650™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 510™ anti-human CD366 (Tim-3) F38-2E2 FC
PE/Dazzle™ 594 anti-human CD366 (Tim-3) F38-2E2 FC
GoInVivo™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC/Fire™ 750 anti-human CD366 (Tim-3) F38-2E2 FC
Pacific Blue™ anti-human CD366 (Tim-3) F38-2E2 FC
Biotin anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-A0169 anti-human CD366 (Tim-3) F38-2E2 PG
TotalSeq™-C0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Cyanine5 anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-B0169 anti-human CD366 (Tim-3) F38-2E2 PG
Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody F38-2E2 FC
TotalSeq™-D0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Fire™ 810 anti-human CD366 (Tim-3) F38-2E2 FC
PE/Fire™ 640 anti-human CD366 (Tim-3) F38-2E2 FC
PE/Fire™ 700 anti-human CD366 (Tim-3) F38-2E2 FC
Go To Top Version: 5    Revision Date: 07/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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