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PE anti-mouse CXCL9 (MIG) Antibody

Pricing & Availability
Clone
MIG-2F5.5 (See other available formats)
Regulatory Status
RUO
Other Names
MIG-1, MIG
Isotype
Armenian Hamster IgG
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Product Citations
publications
MIG-2F5_PE_012110
IFN-g-primed (2 hour) and LPS-stimulated (overnight) Balb/c peritoneal macrophages intracellularly stained with MIG-2F5 PE
  • MIG-2F5_PE_012110
    IFN-g-primed (2 hour) and LPS-stimulated (overnight) Balb/c peritoneal macrophages intracellularly stained with MIG-2F5 PE
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515603 25 µg £147
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515604 100 µg £292
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Description

MIG, also known as mig-1, CXCL9, is a member of the alpha subfamily of inflammatory chemokine. It is inducible in macrophages, hepatocytes, and endothelial cells by IFN-γ, but not by TNF-α or bacterial lipopolysacchrides (LPS). Mig functions as a chemotactic factor for resting memory and activated T cells, both CD4+ and CD8+, and natural killer cells. Furthermore, it was reported that Mig induced both calcium signals and chemotaxis in activated B cells and that B cell activation induced expression of mouse CXCR3. MIG and CXCR3 may be important not only to recruit T cells to peripheral inflammatory sites, but also in some cases to maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs to provide optimal humoral responses to pathogens.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Johnson S, et al. 2015. J Virol. 89: 7494-7505. PubMed
  2. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  3. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  4. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  5. Asai A, et al. 2010. Infect Immun. 78:4311. PubMed
  6. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  7. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  8. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
RRID
AB_2245489 (BioLegend Cat. No. 515603)
AB_2245489 (BioLegend Cat. No. 515604)

Antigen Details

Structure
A member of the alpha subfamily of inflammatory chemokine.
Distribution

Inducible in macrophages, hepatocytes, and endothelial cells by IFN-γ.

Function
Recruit T cells to peripheral inflammatory sites, maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs.
Ligand/Receptor
CXCR3
Cell Type
Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Thapa M et al. 2008. J. Immunol. 180(2):1098
2. Whiting D et al. 2004. J. Immunol. 172 (12):7417
3. Helbig KJ. et al. 2009. J Virol. 83(2):836

Gene ID
4283 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CXCL9
Specificity Alt (DOES NOT SHOW ON TDS):
CXCL9
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC
UniProt
View information about CXCL9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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