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PE/Cyanine7 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_PECy7_110210
C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cyanine7.
  • PK136_PECy7_110210
    C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cyanine7.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
108713 25 µg £60
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108714 100 µg £136
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  2. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  3. Li X, et al. 2022. J Clin Invest. 132: . PubMed
  4. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  5. Fu A, et al. 2022. Cell. 185:1356. PubMed
  6. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  7. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  8. An X, et al. 2023. Am J Reprod Immunol. 89:e13676. PubMed
  9. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  10. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  11. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  12. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  13. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  14. He Y, et al. 2023. Biomolecules. 13:. PubMed
  15. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  16. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  17. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  18. Du H et al. 2019. Cancer cell. 35(2):221-237 . PubMed
  19. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  20. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  21. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  22. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  23. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  24. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  25. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  26. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  27. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  28. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  29. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  30. Yang X, et al. 2019. Oncotarget. 10:4180. PubMed
  31. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  32. Robbins G, et al. 2012. J Biol Chem. 287:24294. PubMed
  33. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  34. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  35. Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed
  36. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  37. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  38. Hutter K, et al. 2020. FEBS J. . PubMed
  39. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  40. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  41. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  42. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  43. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  44. Wu B, et al. 2020. Int J Biol Sci. 16:1526. PubMed
  45. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  46. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  47. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  48. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
  49. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  50. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  51. Itoga M, et al. 2015. PLoS One. 10: e0123210. PubMed
  52. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  53. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  54. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  55. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  56. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  57. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  58. Verma M, et al. 2021. J Exp Med. 218:. PubMed
  59. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
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RRID
AB_389363 (BioLegend Cat. No. 108713)
AB_389363 (BioLegend Cat. No. 108714)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
NK-1.1
Specificity Alt (DOES NOT SHOW ON TDS):
NK-1.1
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about NK-1.1 on UniProt.org

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 2    Revision Date: 12/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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