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Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) Antibody

Pricing & Availability
Clone
N-GAD65 (See other available formats)
Regulatory Status
RUO
Other Names
Glutamate Decarboxylase 2, GAD65, Glutamate Decarboxylase 65 kD Isoform, GABA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N-GAD65_PURE_GAD65_Antibody_1_WB_052118
Western blot of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) antibody (clone N-GAD65). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N-GAD65_PURE_GAD65_Antibody_1_WB_052118
    Western blot of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) antibody (clone N-GAD65). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N-GAD65_PURE_GAD65_Antibody_2_052118
    IHC staining of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N-GAD65_PURE_GAD65_Antibody_3_052118
    IHC staining of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N-GAD65_PURE_GAD65_Antibody_4_052118
    IHC staining of purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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844502 100 µg £189
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Description

GAD gene encodes one of the several forms of glutamic acid decarboxylase, which is identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid (GABA) from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. GAD65 targets presynaptic clusters in axons and exhibits relative dendritic exclusion. GAD65 synthesizes GABA for neurotransmission and is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter (VGAT), which, as a complex, helps package GABA into vesicles for release during neurotransmission.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide immunogen corresponding to amino acids 4-22 of human GAD65.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry formalin-fixed paraffin-embedded tissues, a concentration range of 1.0 - 5.0 µg/ml is suggested.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation.1

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844503) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This antibody demonstrates weaker reactivity to human compared to mouse GAD65/GAD2

Application References

(PubMed link indicates BioLegend citation)
  1. Hampe CS, et al. 2001. J. Neuroimmunol. 113:63. (IP, WB)
  2. Hampe CS, et al. 2007. Clin. Exp. Immunol. 148:72.
  3. Ziegler B, et al. 1996. Acta Diabetol. 33:225.
  4. Raju R, et al. 2005. J. Immunol. 175:7755.
RRID
AB_2565802 (BioLegend Cat. No. 844502)

Antigen Details

Structure
585 amino acids with a predicted molecular weight of 65 kD.
Distribution

Cytoplasm, cytoplasmic vesicle, golgi apparatus, plasma membrane, and marker of pre-synaptic terminals.

Function
Catalyzes production of GABA from L-glutamic acid.
Interaction
Taurine and hypotaurine metabolism, transmission across chemical synapses.
Cell Type
GABAergic Neurons
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References

1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.

Gene ID
2572 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Glutamic Acid Decarboxylase
Specificity Alt (DOES NOT SHOW ON TDS):
Glutamic Acid Decarboxylase (GAD65)
App Abbreviation (DOES NOT SHOW ON TDS):
WB,IHC-P,IP
UniProt
View information about Glutamic Acid Decarboxylase on UniProt.org
Go To Top Version: 2    Revision Date: 05/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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