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Purified anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B4-1_Purified_071007
PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with purified 4B4-1, followed by biotinylated anti-mouse IgG and Sav-PE
  • 4B4-1_Purified_071007
    PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with purified 4B4-1, followed by biotinylated anti-mouse IgG and Sav-PE
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309802 100 µg £90
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Law JC, et al. 2021. J Immunol. 206:37. PubMed
  2. Lu X, et al. 2021. J Exp Med. 218: . PubMed
  3. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  4. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  5. Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed
  6. Sun R, et al. 2022. Cells. 11:. PubMed
  7. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  8. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  9. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  10. Tang JS, et al. 2020. Food Funct. 11:5782. PubMed
  11. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  12. Ichikawa J, et al. 2020. Clin Cancer Res. 26:3384. PubMed
  13. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  14. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID
AB_314781 (BioLegend Cat. No. 309802)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD137
Specificity Alt (DOES NOT SHOW ON TDS):
CD137
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IP,ELISA
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 2    Revision Date: 08/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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