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Purified anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Pure_020608
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • 6D5_Pure_020608
    C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
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115501 50 µg £29
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115502 500 µg £85
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  2. Tersteegen A, et al. 2021. Infect Immun. 89:. PubMed
  3. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  4. Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed
  5. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  6. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  7. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  8. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  9. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  10. Yuan X, et al. 2023. J Periodontol. 94:277. PubMed
  11. Fernandes V, et al. 2020. PLoS Pathog. 16:e1008464. PubMed
  12. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  13. Boal-Carvalho I, et al. 2020. EMBO Rep. 21:e50421. PubMed
  14. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  15. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  16. Field CS, et al. 2018. Oncoimmunology. 7:e1376154. PubMed
  17. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  18. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  19. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  20. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  21. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  22. Taylor NA, et al. 2017. J Clin Invest. 127:3472. PubMed
  23. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  24. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  25. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  26. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  27. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  28. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
  29. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  30. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  31. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  32. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  33. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  34. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  35. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  36. Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed
  37. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  38. Majer O, et al. 2019. Nature. 575:366. PubMed
  39. You X, et al. 2022. Front Genet. 12:790990. PubMed
  40. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  41. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  42. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  43. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  44. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  45. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  46. Rigo MM, et al. 2020. FEBS J. :. PubMed
  47. Seo SU, et al. 2021. Front Immunol. 12:697162. PubMed
  48. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  49. Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed
  50. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  51. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
  52. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  53. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  54. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  55. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  56. Penninger T 2012. J Immunol. 188:1201. PubMed
  57. Korin B, et al. 2020. Sleep. :43. PubMed
  58. Hayashida K, et al. 2008. J Biol Chem. 283:19895. PubMed
  59. Stevens MY, et al. 2020. J Neuroinflammation. 0.899305556. PubMed
  60. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  61. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  62. Atif SM, et al. 2019. JCI Insight. 4:e125494. PubMed
  63. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  64. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  65. Stadnisky M, et al. 2011. Blood. 117:5133. PubMed
  66. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  67. Hwang JY, et al. 2020. Front Immunol. 11:570661. PubMed
  68. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  69. Chen YQ, et al. 2019. J Immunother Cancer. 0.415277778. PubMed
RRID
AB_313636 (BioLegend Cat. No. 115501)
AB_313636 (BioLegend Cat. No. 115502)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD19
Specificity Alt (DOES NOT SHOW ON TDS):
CD19
App Abbreviation (DOES NOT SHOW ON TDS):
FC,CyTOF®,IHC-F
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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