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Purified anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
FA-11_A647_021810
Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647
  • FA-11_A647_021810
    Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647
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137001 50 µg £70
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137002 500 µg £294
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
IP, WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Milasan A, et al. 2017. J Am Heart Assoc. 10.1161/JAHA.117.006892. PubMed
  2. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
  3. Miho Sato‐Hashimoto et al. 2019. eLife. 8 pii: e42025. PubMed
  4. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  5. Zaman R, et al. 2021. Immunity. :. PubMed
  6. Fang W, et al. 2020. Transl Res. 222:28. PubMed
  7. Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed
  8. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  9. Mathew DJ, et al. 2021. Sci Rep. 11:22880. PubMed
  10. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  11. Meng J, et al. 2022. J Neurosci. 42:4958. PubMed
  12. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  13. Block CL, et al. 2022. Cell Rep. 40:111161. PubMed
  14. Saraswathula A, et al. 2023. Int Forum Allergy Rhinol. 13:230. PubMed
  15. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  16. Ohkubo R, et al. 2022. Cell Rep. 41:111803. PubMed
  17. Wu J, et al. 2023. Cell Mol Biol Lett. 28:11. PubMed
  18. , et al. 2022. JACC Basic Transl Sci. 7:I. PubMed
  19. Ruan S, et al. 2023. Front Pharmacol. 14:1088957. PubMed
  20. Michurina S, et al. 2023. Int J Mol Sci. 24:. PubMed
  21. Ritzel RM, et al. 2023. Sci Adv. 9:eadd1101. PubMed
  22. Yin XY, et al. 2023. J Neuroinflammation. 20:69. PubMed
  23. Rodriguez Y, et al. 2022. Liver Res. 6:227. PubMed
  24. Zhang R, et al. 2023. Stroke Vasc Neurol. . PubMed
  25. Dietsch B, et al. 2023. BMC Cancer. 23:436. PubMed
  26. Schake MA, et al. 2023. iScience. 26:106760. PubMed
  27. Taniguchi M, et al. 2023. Mol Pharmacol. . PubMed
  28. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  29. Guimbal S, et al. 2021. Arterioscler Thromb Vasc Biol. 41:e193. PubMed
  30. McKinstry S, et al. 2014. J Neurosci. 34:9455. PubMed
  31. Spencer WJ, et al. 2019. Proc Natl Acad Sci U S A. 116:13087. PubMed
  32. Henjum K, et al. 2020. Sci Rep. 10:10590. PubMed
  33. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  34. Sherafat A, et al. 2021. Nat Commun. 12:2265. PubMed
  35. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  36. Gomez-Pastor R, et al. 2017. Nat Commun. 8:14405. PubMed
  37. Trus I, et al. 2020. Front Immunol. 2.553472222. PubMed
  38. Shen L et al. 2019. Br J Pharmacol. 176(14):2559-2572 . PubMed
  39. Brocker CN, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 312:G283. PubMed
  40. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  41. Burrus CJ, et al. 2020. Cell Rep. 30:642. PubMed
  42. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  43. Lyras EM, et al. 2022. Elife. 11: . PubMed
  44. Batool M, et al. 2020. Basic Res Cardiol. 115:68. PubMed
  45. Mariot V, et al. 2021. J Cachexia Sarcopenia Muscle. Online ahead of print. PubMed
  46. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  47. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  48. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  49. Mori H, et al. 2015. Toxicol Pathol. 43: 883-889. PubMed
  50. Silburt J, et al. 2022. J Neuroinflammation. 19:24. PubMed
  51. Kim H, et al. 2020. ACS Nano. 14:6519. PubMed
  52. Carvalho K, et al. 2022. J Neuroinflammation. 19:178. PubMed
  53. Stafeev I, et al. 2022. Front Nutr. 8:809732. PubMed
  54. Shi W, et al. 2021. Biosci Rep. 41:. PubMed
  55. Goldblatt DL, et al. 2020. British Journal of Pharmacology. 177(10):2256-2273.. PubMed
  56. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  57. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  58. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  59. Wu Y, et al. 2019. Nat Commun. 10:58. PubMed
  60. Cornelison RC, et al. 2022. NPJ Precis Oncol. 6:54. PubMed
  61. Choi SH, et al. 2020. Arterioscler Thromb Vasc Biol. :ATVBAHA120315485. PubMed
  62. Wang L, et al. 2020. Sci Rep. 10:16326. PubMed
  63. Collier AF, et al. 2018. J Pharmacol Exp Ther. 364:409. PubMed
  64. Cushing EM, et al. 2018. J Lipid Res. 59:1230. PubMed
  65. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  66. Koch PS, et al. 2021. Front Physiol. 12:722394. PubMed
  67. Milasan A, et al. 2019. Atherosclerosis. 283:106. PubMed
  68. Phua T, et al. 2017. Sci Rep. 10.1038/srep44351. PubMed
  69. Cheng X, et al. 2021. Redox Biol. 40:101863. PubMed
  70. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  71. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  72. Dong Y, et al. 2021. STAR Protoc. 2:100853. PubMed
  73. Ni K, et al. 2018. Respir Res. 0.865972222. PubMed
  74. Yue F, et al. 2021. Mol Ther. 29:132. PubMed
  75. Han X, et al. 2020. Mol Carcinog. 59:1088. PubMed
  76. Healy A, et al. 2020. Arterioscler Thromb Vasc Biol. 40:714. PubMed
  77. Kayama H, et al. 2012. Proc Natl Acad Sci U S A. 109:5010. PubMed
RRID
AB_2044003 (BioLegend Cat. No. 137001)
AB_2044003 (BioLegend Cat. No. 137002)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD68
Specificity Alt (DOES NOT SHOW ON TDS):
CD68
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC,FC,IP,WB,IHC-F
UniProt
View information about CD68 on UniProt.org
Go To Top Version: 2    Revision Date: 04/26/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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