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ELISA MAX™ Deluxe Set Rat TNF-a

Pricing & Availability
Regulatory Status
RUO
Other Names
Tumor Necrosis Factor α (TNF-α) ELISA Kit, Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF)
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Product Citations
publications
TNF-alpha_Rat_052511
  • TNF-alpha_Rat_052511
Cat # Size Price Quantity Check Availability Save
438204 5 plates £238
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Description

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TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing.  Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.  It has been shown that TNF-α plays a critical role in many physiological and pathological conditions including neurodegenerative diseases and cancer.

This kit is specifically designed for the accurate quantitation of Rat TNF-α from cell culture supernatant, serum, plasma, or other biological fluids.  It is designed to meet the increasing use of rat models in biomedical studies.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Rat TNF-α ELISA MAX™ Capture Antibody (200X)
  • Rat TNF-α ELISA MAX™ Detection Antibody (200X)
  • Rat TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
  • Matrix Diluent A (For serum and plasma samples only)

Product Details

Verified Reactivity
Rat
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Wahba NS, et al. 2022. Fundam Clin Pharmacol. 36:306. PubMed
  2. da Silva TP, et al. 2022. Cell Biochem Funct. :. PubMed
  3. Asaad GF, et al. 2022. Iran J Basic Med Sci. 25:1522. PubMed
  4. Hajighorbani M, et al. 2017. Inflammopharmacology. 10.1007/s10787-017-0335-2. PubMed
  5. Garcia-Cortadella R, et al. 2021. Nat Commun. 0.646527778. PubMed
  6. Schmidt K, et al. 2017. Microvasc Res. 10.1016/j.mvr.2017.08.002. PubMed
  7. Sanchez TW, et al. 2020. Am J Reprod Immunol. 83:e13205. PubMed
  8. van der Merwe Y, et al. 2019. Sci Rep. 9:3482. PubMed
  9. Lai PH, et al. 2021. J Neuroinflammation. 18:35. PubMed
  10. Murugesan P, et al. 2016. J Infect Dis. 213: 532 - 540. PubMed
  11. Niu Q, et al. 2022. Life (Basel). 12:. PubMed
  12. Lotter J, et al. 2020. Front Psychiatry. 11:121. PubMed
Sensitivity
2 pg/ml
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
24835 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
TNF-alpha
Specificity Alt (DOES NOT SHOW ON TDS):
TNF-α
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 09/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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