The Notch receptors are highly conserved from invertebrates to mammals.While Notch1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences.The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus.This translocation triggers a series of signaling process.Notch 3 is primarily expressed in adult arterial smooth muscle cells.Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution
Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.
Function
Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
Ligand/Receptor
Jag1,2; Delta 1,2
Cell Type
Epithelial cells
Biology Area
Immunology
Antigen References
1. Ehebauer ME et al. 2006. Biochem J 392:13 2. Shimizu K et al. 2000. Mol Cell Biology 20:18 3. Tanigaki K et al. 2007. Nature Immunol 8:451 4. Bellavia D et al. 2008. 27:5092 5. Louvi A et al.Dev Neurosci. 2006. 28:5
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