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Biotin anti-human CD63 Antibody

Pricing & Availability
Clone
H5C6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H5C6_Biotin_CD63_Antibody_FC_110512
Thrombin-activated platelets were stained with biotinylated CD63 (clone H5C6, filled histogram) or mouse IgG1, κ (open histogram) isotype control, followed by Sav-PE.
  • H5C6_Biotin_CD63_Antibody_FC_110512
    Thrombin-activated platelets were stained with biotinylated CD63 (clone H5C6, filled histogram) or mouse IgG1, κ (open histogram) isotype control, followed by Sav-PE.
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353017 50 µg £61
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353018 200 µg £182
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Description

CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T cell line HPB-ALL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
  2. Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
Product Citations
  1. Kolan S, et al. 2015. Biochem Biophys Res Commun. 460:645. PubMed
  2. Suthar J, et al. 2023. Nanoscale Horiz. 8:460. PubMed
  3. Yildizhan Y, et al. 2023. Int J Mol Sci. 24:. PubMed
  4. Theodoraki MN, et al. 2023. Oncol Lett. 25:200. PubMed
  5. Yildizhan Y, et al. 2021. J Extracell Vesicles. e12059:10. PubMed
  6. Suthar J, et al. 2020. Anal Chem. 92:4082. PubMed
  7. Theodoraki MN, et al. 2018. Clin Cancer Res. 24:896. PubMed
  8. Wang J, et al. 2020. Sci Adv. 6:eaax3223. PubMed
  9. Theodoraki MN, et al. 2020. Oncoimmunology. 9:1747732. PubMed
  10. Kim D, et al. 2016. Proc Natl Acad Sci U S A. 113: 170 - 175. PubMed
  11. Zheng W, et al. 2022. Nat Biomed Eng. 6:979. PubMed
  12. Turiello R, et al. 2022. J Immunother Cancer. 10:. PubMed
  13. Theodoraki MN, et al. 2021. Br J Cancer. 125:1677. PubMed
  14. Köksal AR, et al. 2022. J Hepatocell Carcinoma. 9:959. PubMed
  15. Priglinger E, et al. 2021. J Extracell Vesicles. 10:e12156. PubMed
  16. Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed
  17. Mondal SK, et al. 2021. Methods Mol Biol. 2265:305. PubMed
  18. Hofmann L, et al. 2020. Int J Mol Sci. 21:00. PubMed
RRID
AB_2561676 (BioLegend Cat. No. 353017)
AB_2561676 (BioLegend Cat. No. 353018)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Azorsa DO, et al. 1991. Blood 78:280.
  2. Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
  3. Hildreth JE, et al. 1991. Blood 77:121.
  4. Anzai N, et al. 2002. Blood 99:4413.
Gene ID
967 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD63
Specificity Alt (DOES NOT SHOW ON TDS):
CD63
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD63 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 03/27/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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