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Brilliant Violet 421™ anti-human CD183 (CXCR3) Antibody

Pricing & Availability
Clone
G025H7 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2
Isotype
Mouse IgG1, κ
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Product Citations
publications
G025H7_BV421_1_021512
Human peripheral blood lymphocytes were stained with CD3 FITC and CXCR3 (clone G025H7) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • G025H7_BV421_1_021512
    Human peripheral blood lymphocytes were stained with CD3 FITC and CXCR3 (clone G025H7) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • G025H7_BV421_2_021512
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
353715 25 tests £140
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353716 100 tests £279
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Description

Human CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR3 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Rosenberg JM, et al. 2022. Med (N Y). 3:42. PubMed
  2. Breznik JA, et al. 2022. J Immunol. 209:1892. PubMed
  3. Woodcock V, et al. 2023. BJUI Compass. 4:322. PubMed
  4. Bhatia V, et al. 2023. Nat Commun. 14:2041. PubMed
  5. Imai H, et al. 2023. iScience. 26:106822. PubMed
  6. Apostolidis SA, et al. 2021. Nat Med. 27:1990. PubMed
  7. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  8. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  9. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  10. Bouwer A, et al. 2014. J Immunol. 192:2514. PubMed
  11. Ma C, et al. 2015. J Allergy Clin Immunol. . PubMed
  12. Ahmed R, et al. 2020. Cell Rep. 33:108501. PubMed
  13. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  14. Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed
  15. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  16. Painter MM, et al. 2021. Immunity. 54:2133. PubMed
  17. Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed
  18. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  19. Qu K et al. 2017. Cancer cell. 32(1):27-41 . PubMed
  20. NULL, et al. 2022. Cell. 185:916. PubMed
  21. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  22. Lederer K, et al. 2022. Cell. . PubMed
  23. Pandya J, et al. 2016. J Leukoc Biol. 100: 823 - 833. PubMed
  24. Adel–Patient K, et al. 2018. Clin Transl Allergy. 8:38. PubMed
  25. Yost KE, et al. 2019. Nat Med. 25:1251. PubMed
RRID
AB_11124720 (BioLegend Cat. No. 353715)
AB_11124720 (BioLegend Cat. No. 353716)

Antigen Details

Structure
CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor
Distribution

T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells

Function
Essential in T cell recruitment to sites of inflammation
Ligand/Receptor
CXCL9, CXCL10, and CXCL11
Cell Type
Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Loetscher M, et al. 1996. J. Exp. Med. 184:963.
2. Cole KE, et al. 1998. J. Exp. Med. 187:2009.
3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909.
4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887.
5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089.
6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67.

Gene ID
2833 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD183
Specificity Alt (DOES NOT SHOW ON TDS):
CD183
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD183 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD183 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD183 (CXCR3) G025H7 FC
APC/Cyanine7 anti-human CD183 (CXCR3) G025H7 FC
FITC anti-human CD183 (CXCR3) G025H7 FC
PE anti-human CD183 (CXCR3) G025H7 FC
APC anti-human CD183 (CXCR3) G025H7 FC
Alexa Fluor® 488 anti-human CD183 (CXCR3) G025H7 FC
Alexa Fluor® 647 anti-human CD183 (CXCR3) G025H7 FC
PerCP/Cyanine5.5 anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 421™ anti-human CD183 (CXCR3) G025H7 FC
PE/Cyanine7 anti-human CD183 (CXCR3) G025H7 FC
Pacific Blue™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 510™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 605™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 650™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 711™ anti-human CD183 (CXCR3) G025H7 FC
Purified anti-human CD183 (CXCR3) (Maxpar® Ready) G025H7 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD183 (CXCR3) G025H7 FC
PerCP anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 785™ anti-human CD183 (CXCR3) G025H7 FC
Alexa Fluor® 700 anti-human CD183 (CXCR3) G025H7 FC
Biotin anti-human CD183 (CXCR3) G025H7 FC
TotalSeq™-A0140 anti-human CD183 (CXCR3) G025H7 PG
TotalSeq™-C0140 anti-human CD183 (CXCR3) G025H7 PG
Ultra-LEAF™ Purified anti-human CD183 (CXCR3) G025H7 Block,FC
TotalSeq™-B0140 anti-human CD183 (CXCR3) G025H7 PG
APC/Fire™ 750 anti-human CD183 (CXCR3) G025H7 FC
TotalSeq™-D0140 anti-human CD183 (CXCR3) G025H7 PG
PE/Fire™ 810 anti-human CD183 (CXCR3) Antibody G025H7 FC
APC/Fire™ 810 anti-human CD183 (CXCR3) Antibody G025H7 FC
PE/Cyanine5 anti-human CD183 (CXCR3) Antibody G025H7 FC
PE/Fire™ 640 anti-human CD183 (CXCR3) G025H7 FC
PerCP/Fire™ 780 anti-human CD183 (CXCR3) G025H7 FC
Spark Red™ 718 anti-human CD183 (CXCR3) (Flexi-Fluor™) G025H7 FC
PE/Fire™ 700 anti-human CD183 (CXCR3) G025H7 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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