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Brilliant Violet 421™ anti-human CD86 Antibody

Pricing & Availability
Clone
BU63 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
B7-2, B70, Ly-58
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BU63_BV421_CD86_Antibody_032918
Human peripheral blood monocytes stained with CD14 APC and CD86 (clone BU63, left) Brilliant Violet 421™ or Mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
  • BU63_BV421_CD86_Antibody_032918
    Human peripheral blood monocytes stained with CD14 APC and CD86 (clone BU63, left) Brilliant Violet 421™ or Mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
374211 25 tests £140
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374212 100 tests £275
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ARH 77 (B lymphoblastoid cell line).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  2. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  3. Ji K, et al. 2023. Clin Transl Med. 13:e1234. PubMed
  4. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
RRID
AB_2728393 (BioLegend Cat. No. 374211)
AB_2728393 (BioLegend Cat. No. 374212)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T Cell activation
Interaction
C-Jun, NFκB
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131. 
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD86
Specificity Alt (DOES NOT SHOW ON TDS):
CD86
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD86 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 03/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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