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HRP Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Horseradish Peroxidase, SAv-HRP
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405210 1 mL £89
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation
Streptavidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling
The streptavidin-HRP solution should be stored undiluted between 2°C and 8°C. Avoid exposure to sodium azide.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
ELISPOT, WB, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this Streptavidin-HRP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:3000 to determine optimal conditions. For ELISPOT analysis, the reagent should be titrated between 1:500 - 1:1500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.

Application References

(PubMed link indicates BioLegend citation)
  1. Li KJ, et al. 2006. J. Leukoc. Biol. doi:10.1189/jlb.1105668.PubMed
  2. Weldon S, et al. 2009. J. Immunol. 183:8148. PubMed
  3. Basnayake K, et al. 2010. J Am Soc Naphrol. 21:1165. PubMed
  4. Marder W, et al. 2011. Ann Rheum Dis. PubMed
  5. Cheng J, et al. 2012. Mol Biol Cell. 23:2891. PubMed
  6. Vishwakarma V, et al. 2012. Infect Immun. 80:3236. PubMed
  7. Sun X, et al. 2013. J. Immunol. 190:2536. PubMed
  8. Van Phan T, et al. 2013. Biochem Pharmacol. 85:1145. PubMed
  9. Ranganathan P, et al. 2013. J Rhematol. 40:129. PubMed
  10. Din Su, et al. 2013. Occup Med. PubMed
  11. Bulut GB, et al. 2013. Blood. 122:3964. PubMed
  12. Chia YL, et al. 2013. Antiviral Res. 3542:354. PubMed
  13. Ahn JM, et al. 2014. Mol Cell Proteomics. 13:30. PubMed
  14. Din SU, et al. 2014. Occup Med. 64:39. PubMed
  15. Balmer ML, et al. 2014. Sci Transl Med. 21:237. PubMed
  16. Klehmet J, et al. 2014. J Neurol Neurosurg Psychiatry. PubMed
  17. Wu CC, et al. 2014. Cancer Epidemiol. Biomarkers Prev. 23:1569. PubMed
  18. Peng JG, et al. 2014. J Virol. 88:8386. PubMed
  19. Chlon TM, et al. 2014. J Virol. 88:11315. PubMed
  20. Sadovskaya I, et al. 2014. Carbohydr Poly. 111:139. PubMed
  21. Liu T, et al. 2015. Infect Immun. 83:2011. PubMed
  22. Himmelein S, et al. 2015. J Virol. 89:5747. PubMed
  23. Dinter J, et al. 2015. J Mol Endocrinol. 54:205. PubMed
  24. Yang L, et al. 2015. J Immunol. 194:5635. PubMed
Product Citations
  1. Zhang C, et al. 2015. J Surgical Res. 197: 301-306. PubMed
  2. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  3. Wang YH, et al. 2018. Oncol Lett. 16:6903. PubMed
  4. Csobán-Szabó Z, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Lopez-Gordo E, et al. 2022. Front Med (Lausanne). 8:732095. PubMed
  6. Tabynov K, et al. 2022. NPJ Vaccines. 7:24. PubMed
  7. Clemente V, et al. 2022. Cancer Res Commun. 2:784. PubMed
  8. Laver JR, et al. 2021. Sci Transl Med. 13: . PubMed
  9. Wang Q, et al. 2022. EMBO J. 41:e110636. PubMed
  10. Johnson BS, et al. 2022. Mol Cell Proteomics. 21:100256. PubMed
  11. Ye D, et al. 2022. J Pineal Res. 73:e12828. PubMed
  12. Gao SS, et al. 2022. iScience. 25:105481. PubMed
  13. He Q, et al. 2023. Life Sci Alliance. 6: . PubMed
  14. Cho YK, et al. 2022. Sci Adv. 8:eabq4022. PubMed
  15. Qin J, et al. 2023. Front Immunol. 14:1126392. PubMed
  16. Watanabe T, et al. 2020. Cytotherapy. . PubMed
  17. Gendron D, et al. 2017. Pulm Pharmacol Ther. 10.1016/j.pupt.2017.03.010. PubMed
  18. Kongsuphol P, et al. 2022. Commun Med (Lond). 1:46. PubMed
  19. Cheng J, et al. 2012. Mol Biol Cell. 23:2891. PubMed
  20. Tu S, et al. 2015. Nature. 534:387-390. PubMed
  21. Brugiroux S, et al. 2016. Nat Microbiol. 2:16215. PubMed
  22. Kikuchi N, et al. 2019. Biol Pharm Bull. 42:57. PubMed
  23. Li Q, et al. 2020. Cell Stem Cell. 675:26. PubMed
  24. Himmelein S, et al. 2015. J Virol. 89:5747. PubMed
  25. Ahn J, et al. 2014. Mol Cell Proteomics. 13:30. PubMed
  26. Gamache J, et al. 2019. Nat Commun. 10:2479. PubMed
  27. Hooper K, Kong W, Ganea D, et al. 2017. PLoS One. 12(6):e0179184. PubMed
  28. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  29. Fan X, et al. 2021. Int J Mol Sci. 22:. PubMed
  30. Wu J 2014. J Virol. 88:8386. PubMed
  31. Cárdenas–Torres FI, et al. 2019. Medicina (Kaunas). 55:3. PubMed
  32. Duan S, et al. 2019. J Clin Invest. 129:1387. PubMed
  33. Sielemann J, et al. 2021. Nat Commun. 12:6549. PubMed
  34. Hwang D, et al. 2016. PLoS One. 11: 0152522. PubMed
  35. Marder W, et al. 2011. Ann Rheum Dis. 70:1550. PubMed
  36. Li K, et al. 2006. J Leukoc Biol . 80:350. PubMed
  37. Asarnow D, et al. 2021. Cell. 184:3192. PubMed
  38. Vishwakarma V, et al. 2012. Infect Immun . 80:3236. PubMed
  39. Nakanishi M, et al. 2019. Cell. 177:910. PubMed
  40. Czakai K, et al. 2016. Sci Rep. 6:27990. PubMed
  41. Weldon S, et al. 2009. J Immunol. 183:8148. PubMed
  42. Duan S, et al. 2021. J Immunol. 206:2290. PubMed
  43. Phillips AT, et al. 2021. Invest Ophthalmol Vis Sci. 62:15. PubMed
  44. Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed
  45. Peng Y 2017. PLoS One. 10.1371/journal.pone.0188112. PubMed
  46. Morita M, et al. 2021. Heliyon. 7:e06228. PubMed
  47. MÜller A, et al. 2016. PLoS One. 11:e0168260. PubMed
  48. Tidley S 2014. Occup Med. 64:39. PubMed
  49. Ye F, et al. 2016. J Virol. 90: 9654 - 9663. PubMed
  50. Crowley SJ, et al. 2020. Open Biol. 10:190235. PubMed
  51. Morgan R, et al. 2016. PLoS One. 11: 0162008. PubMed
  52. Chlon T, et al. 2014. J Virol. 88:11315. PubMed
  53. Tuladhar R, et al. 2019. J Biol Chem. 294:6273. PubMed
  54. Gröschel C, et al. 2017. Sci Rep.. 10.1038/s41598-017-16147-1. PubMed
  55. Tsukamoto H, et al. 2017. FEBS Lett. 10.1002/1873-3468.12768. PubMed
  56. Shukla SP, et al. 2021. Int J Mol Sci. 22:. PubMed
  57. Simic MS, et al. 2019. Sci Adv. 5:eaaw0025. PubMed
  58. Herp S, et al. 2019. Cell Host Microbe. 25:681. PubMed
  59. Vijver SV, et al. 2021. Front Immunol. 12:733561. PubMed
  60. Cildag S, et al. 2021. Med Pharm Rep. 94:53. PubMed
  61. Bello A, et al. 2021. Immunol Cell Biol. 99:879. PubMed
  62. Klehmet J, et al. 2014. J Neurol Psychiatry. . PubMed
  63. Chia Y, et al. 2013. Antiviral Res. 3542:354. PubMed
  64. Bulut G, et al. 2013. Blood. 122:3964. PubMed
  65. den Hartigh AB, et al. 2018. Curr Protoc Immunol. :e52. PubMed
  66. Clemente V, et al. 2021. Cells. 10: . PubMed
  67. Yen J, et al. 2015. J Leukoc Biol. 98: 689-702. PubMed
  68. Ozawa T, et al. 2021. iScience. 24:102488. PubMed
  69. Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed
  70. Tsanov K, et al. 2016. Nat Cell Biol. 19:60-67. PubMed
  71. Baerenwaldt A, et al. 2016. J Immunol. 196: 2561 - 2571. PubMed
  72. Dinter J, et al. 2015. J Mol Endocrinol. 54:205. PubMed
  73. Makowski EK, et al. 2021. MAbs. 13:1951426. PubMed
  74. Sun X, et al. 2013. J Immunol. 190:2536. PubMed
  75. Balmer M, et al. 2014. Sci Transl Med. 21:237. PubMed
  76. Wu C, et al. 2014. Cancer Epidemiol Biomarkers Prev. 23:1569. PubMed
  77. Kiriakidis S, et al. 2017. Kidney International. 10.1016/j.kint.2017.03.008. PubMed
  78. Basnayake K, et al. 2010. J Am Soc Nephrol. 1.684027778. PubMed
  79. Kim DJ, et al. 2020. Proc Natl Acad Sci U S A. 117:15837. PubMed
  80. Parrish H, et al. 2016. Proc Natl Acad Sci U S A. 113: 3000 - 3005. PubMed
  81. Ugurlu-Çimen D, et al. 2021. Epigenetics Chromatin. 14:32. PubMed
  82. Phan T, et al. 2013. Biochem Pharmacol. 85:1145. PubMed
  83. Sadovskaya I, et al. 2014. Carbohydr Polym. 111:139. PubMed
  84. Xing QR, et al. 2020. Sci Adv. 6:00. PubMed
  85. von Wenserski L, et al. 2020. Leukemia. . PubMed
  86. Isernhagen A, et al. 2015. EMBO Mol Med. 7: 1480 - 1502. PubMed
  87. Shinohara M 2015. J Immunol. 194:5635. PubMed
  88. Liu T, et al. 2015. Infect Immun . 83:2011. PubMed
  89. Gröschel C, et al. 2018. Front Immunol. 9:2665. PubMed
  90. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed

Antigen Details

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
Biotin
Specificity Alt (DOES NOT SHOW ON TDS):
Biotin
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA,ELISPOT,IHC,WB
UniProt
View information about Biotin on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 12/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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