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PE anti-rat IgG2a Antibody

Pricing & Availability
Clone
MRG2a-83 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Mouse IgG
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Product Citations
publications
MRG2a-83_PE_IgG2a_Antibody_FC_102714
C57BL/6 mouse splenocytes were stained with purified CD19 followed by anti-rat IgG2a (clone MRG2a-83) PE.
  • MRG2a-83_PE_IgG2a_Antibody_FC_102714
    C57BL/6 mouse splenocytes were stained with purified CD19 followed by anti-rat IgG2a (clone MRG2a-83) PE.
Compare all formats See PE spectral data
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407507 50 µg £72
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407508 200 µg £180
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Description

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
Product Citations
  1. Gall CLM, et al. 2023. Bio Protoc. 13:e4613. PubMed
  2. Huber M, et al. 2023. Nat Commun. 14:2143. PubMed
  3. Banka AL, et al. 2023. Nat Commun. 14:2462. PubMed
  4. Yang Z, et al. 2020. J Invest Dermatol. 544:140. PubMed
  5. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  6. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
RRID
AB_2564487 (BioLegend Cat. No. 407507)
AB_2564487 (BioLegend Cat. No. 407508)

Antigen Details

Gene ID
367586 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgG2a
Specificity Alt (DOES NOT SHOW ON TDS):
IgG2a
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about IgG2a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/03/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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