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Purified anti-human CD14 Antibody

Pricing & Availability
Clone
63D3 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
63D3_PURE_CD14_Antibody_FC_042015
Human peripheral blood monocytes were stained with CD14 (clone 63D3) purified (filled histogram) or mouse IgG1, κ purified (open histogram), followed by anti-mouse IgG FITC.
  • 63D3_PURE_CD14_Antibody_FC_042015
    Human peripheral blood monocytes were stained with CD14 (clone 63D3) purified (filled histogram) or mouse IgG1, κ purified (open histogram), followed by anti-mouse IgG FITC.
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367101 25 µg £37
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367102 100 µg £73
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human peripheral blood monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
  2. Devitt A, et al. 1998. Nature 6675:505.
Product Citations
  1. Huang L, et al. 2020. JCI Insight. 5:00. PubMed
  2. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  3. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  4. Priyanto H, et al. 2021. J Clin Tuberc Other Mycobact Dis. 22:100214. PubMed
  5. Ferdosi SR, et al. 2019. Nat Commun. 1.695833333. PubMed
  6. Bashth OS, et al. 2020. Biomater Sci. 4.128472222. PubMed
  7. Azari S, et al. 2021. MBio. 12:e0184921. PubMed
  8. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  9. Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed
  10. Boulanger MD, et al. 2022. PLoS One. 17:e0269316. PubMed
  11. Chen W, Pilling D, and Gomer R 2017. BMC Immunol. . 10.1186/s12865-017-0230-z. PubMed
RRID
AB_2565627 (BioLegend Cat. No. 367101)
AB_2565627 (BioLegend Cat. No. 367102)

Antigen Details

Structure
GPI-linked membrane glycoprotein.
Distribution

Monocytes, macrophages, dendritic cells, and granulocytes.

Function
LPS receptor, clearance of Gram-negative pathogens.
Interaction
LPS.
Ligand/Receptor
LPS.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD14
Specificity Alt (DOES NOT SHOW ON TDS):
CD14
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 2    Revision Date: 11/14/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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