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Purified anti-human IFN-γ Antibody

Pricing & Availability
Clone
NIB42 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NIB42
Typical standard curves under optimized assay conditions in sandwich assays using two IFNγ antibody pairs with either clone NIB42 or MD-1 as a capture antibody. The detection antibody for both curves was clone 4S.B3.
  • NIB42
    Typical standard curves under optimized assay conditions in sandwich assays using two IFNγ antibody pairs with either clone NIB42 or MD-1 as a capture antibody. The detection antibody for both curves was clone 4S.B3.
Cat # Size Price Quantity Check Availability Save
502401 50 µg £57
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502402 500 µg £161
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 4S.B3 antibody reacts with the human IFN-γ. The NIB42 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli - expressed, recombinant human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, the antibody should be titrated between 0.25 - 2 µg/ml to determine optimal condition. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture2: The purified NIB42 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated 4S.B3 antibody (Cat. No. 502504/502514) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5 - 2.0 µg/ml is recommended.
Neutralization1: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 502405 & 502406).
Note: For testing human IFN-? in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A. 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105.
  2. Goodier M, et al. 2000. J. Immunol. 165:139.
  3. Blank N, et al. 2007. J. Immunol. 179:3613. (Neut)
Product Citations
  1. Yamaguchi YL, et al. 2015. Stem Cells. 289:33. PubMed
  2. Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
  3. Stein N, et al. 2019. iScience. 0.781944444. PubMed
  4. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  5. Kameda M, et al. 2020. PLoS One. 15:e0241719. PubMed
  6. Lockridge J, et al. 2011. PLoS One. 6:e21701. PubMed
RRID
AB_315223 (BioLegend Cat. No. 502401)
AB_315223 (BioLegend Cat. No. 502402)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer, E., et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar, M., et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray, P., et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IFN-gamma
Specificity Alt (DOES NOT SHOW ON TDS):
IFN-γ
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA Capture
UniProt
View information about IFN-gamma on UniProt.org

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IFN-γ NIB42 ELISA Capture
Ultra-LEAF™ Purified anti-human IFN-γ NIB42 ELISA Capture,ELISPOT Capture,Neut
Go To Top Version: 2    Revision Date: 10/07/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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