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- Clone
- 364-3B3-14 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 500104 | 500 µg | £185 | ||||
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The 364-3B3-14 antibody is a non-neutralizing antibody.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human IL-1α
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IHC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Capture1: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.
Flow Cytometry4: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunohistochemistry3. The 364-3B3-14 is a non-neutralizing antibody. -
Application References
(PubMed link indicates BioLegend citation) -
- Thorpe R, et al. 1988. Lymphokine Res. 2:119.
- Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177.
- Lu Y, et al. 2015. PNAS. 112:607. PubMed
- Product Citations
-
- RRID
-
AB_315083 (BioLegend Cat. No. 500104)
Antigen Details
- Structure
- Cytokine; 17.5 kD (Mammalian)
- Bioactivity
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
- Cell Sources
- Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
- Cell Targets
- B cells, T cells, monocytes, dendritic cells
- Receptors
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York.
3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello, C. 1996. Blood 87:2095. - Regulation
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 3552 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IL-1alpha
- Specificity Alt (DOES NOT SHOW ON TDS):
- IL-1α
- App Abbreviation (DOES NOT SHOW ON TDS):
- ELISA Capture,IHC
- UniProt
- View information about IL-1alpha on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All IL-1α Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human IL-1α | 364-3B3-14 | ICFC |
| Purified anti-human IL-1α | 364-3B3-14 | ELISA Capture,IHC |
| Ultra-LEAF™ Purified anti-human IL-1α | 364-3B3-14 | ELISA Capture,ELISPOT Capture,ICFC,IHC |
| TotalSeq™-B1203 anti-human IL-1α | 364-3B3-14 | ICPG |
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human IL-1α
LPS-stimulated (6 hours) human peripheral blood monocytes su... -
Purified anti-human IL-1α
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Ultra-LEAF™ Purified anti-human IL-1α
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TotalSeq™-B1203 anti-human IL-1α
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