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Alexa Fluor® 488 anti-CD68 Antibody

Pricing & Availability
Clone
KP1 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin, CD68 antigen, macrophage antigen CD68, scavenger receptor class D, member 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. KP1_A488_CD68_IHC-P_031125.
IHC staining with Alexa Fluor® 488 anti-CD68 (clone KP1) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline with Tween-20 (Cat. No. 925501), the tissue was incubated without (panel A) and with (panel B) Alexa Fluor® 488 anti-CD68 (clone KP1). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • A. KP1_A488_CD68_IHC-P_031125.
    IHC staining with Alexa Fluor® 488 anti-CD68 (clone KP1) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline with Tween-20 (Cat. No. 925501), the tissue was incubated without (panel A) and with (panel B) Alexa Fluor® 488 anti-CD68 (clone KP1). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • B. KP1_A488_CD68_ICC_031125.
    Jurkat (negative cell line, panels A and C) and THP-1 cells (positive cell line, panels B and D) were fixed and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 488 anti-CD68 (clone KP1). Nuclei were counterstained with DAPI (Cat. No. 422801) and merged (panels A and B). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 488 spectral data
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916108 100 µg £260
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. High levels of CD68 expression are detected in activated microglia. LAMP proteins may have functions related to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Lysosomal fraction of human alveolar macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). Antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) results in correct staining but signal may be dim.

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:

-Fixation buffer (Cat. No. 420801) followed by 100% ice-cold methanol
-100% ice-cold methanol only

It is not recommended to fix/permeabilize using fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100

This product is not recommended for use in intracellular flow cytometry (ICFC).

Application References

(PubMed link indicates BioLegend citation)
  1. Holness CL, et al. 1993. Blood 81(6):1607.
  2. Horny HP, et al. 1990. J. Clin. Path. 43:719.
  3. Pulford KAF, et al. 1989. J. Clin. Path. 42:414.

Antigen Details

Structure
CD68 is a 354 amino acid protein with a predicted molecular weight of 37.4 kD.
Distribution

Microglia, monocytes, macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells

Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Microglia, Monocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Molecular Family
CD Molecules
Antigen References
  1. Holness CL, et al. 1993. Blood. 81(6):1607.
  2. Horny HP, et al. 1990. J Clin Path. 43:719.
  3. Pulford KAF, et al. 1989. J Clin Path. 42:414.
Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/11/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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