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Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac) Antibody

Pricing & Availability
Clone
2G1F9 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
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Product Citations
publications
2G1F9_A488_Histone-H3_Acetylated_Lysine_9_012220.png
Untreated (panel A) and sodium butyrate-treated (5 mM for 16 hours, panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) of Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac) (clone 2G1F9) overnight at 4°C. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
  • 2G1F9_A488_Histone-H3_Acetylated_Lysine_9_012220.png
    Untreated (panel A) and sodium butyrate-treated (5 mM for 16 hours, panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) of Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac) (clone 2G1F9) overnight at 4°C. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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698405 25 µg £94
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698406 100 µg £230
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Description

Histone subunit H3, along with subunits 2A, 2B, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Histone H3 K9ac peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2832850 (BioLegend Cat. No. 698405)
AB_2832850 (BioLegend Cat. No. 698406)

Antigen Details

Structure
Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational.
Distribution

Nucleus

Function
Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones.
Interaction
Histones 2A, 2B, 4, HDAC
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Zhang, et al. 2017. Nat. Commun. 8: 14799.
2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394.
3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333.
4. Li B, et al. 2016. Sci. Rep. 6: 26542.
5. Li D, et al. 2014, Mol. Cancer. 13:26.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 1    Revision Date: 01/22/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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