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Alexa Fluor® 488 anti-human CD147 Antibody

Pricing & Availability
Clone
HIM6 (See other available formats)
Regulatory Status
RUO
Workshop
VI N-L109
Other Names
Neurothelin, Basigin, EMMPRIN
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-HIM6_AF488_071607
Human peripheral blood lymphocytes stained with HIM6 Alexa Fluor® 488
  • a-HIM6_AF488_071607
    Human peripheral blood lymphocytes stained with HIM6 Alexa Fluor® 488
  • b-HIM6_A488_CD147_Antibody_ICC_011921
    HeLa cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of anti-human CD147 (clone HIM6) Alexa Fluor® 488 (green) for 3 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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306207 25 tests £85
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Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Capuchin Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBMCs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell activation2, immunohistochemical staining1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting1. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).

Application References

(PubMed link indicates BioLegend citation)
  1. Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC)
  2. Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
  3. Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
Product Citations
  1. Takatsu H, et al. 2011. J Biol Chem. 286:38159. PubMed
  2. Menck K, et al. 2015. J Mol Cell Biol. 7:143. PubMed
  3. Grass G, et al. 2013. J Biol Chem. 288:26089. PubMed
  4. Naito T, et al. 2015. J Biol Chem. 290:150004. PubMed
RRID
AB_528739 (BioLegend Cat. No. 306207)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution

Leukocytes, erythrocytes, platelets, endothelial cells

Function
Adhesion
Cell Type
Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.

Gene ID
682 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org
Go To Top Version: 5    Revision Date: 01/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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