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Alexa Fluor® 488 anti-human CD285 (TLR5) Antibody

Pricing & Availability
Clone
S16021I (See other available formats)
Regulatory Status
RUO
Other Names
SLE1, TIL3, SLEB1, MELIOS, CD285
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S16021I_A488_CD285_Antibody_Updated_060321.png
Human peripheral blood monocytes were stained with CD14 APC and CD285 (TLR5) (clone S16021I) Alexa Fluor® 488 (left) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (right).
  • S16021I_A488_CD285_Antibody_Updated_060321.png
    Human peripheral blood monocytes were stained with CD14 APC and CD285 (TLR5) (clone S16021I) Alexa Fluor® 488 (left) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (right).
Compare all formats See Alexa Fluor® 488 spectral data
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394509 25 tests £113
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394510 100 tests £257
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Description

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR5 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
RRID
AB_2892462 (BioLegend Cat. No. 394509)
AB_2892462 (BioLegend Cat. No. 394510)

Antigen Details

Structure
Type 1 transmembrane protein, 98 kD
Distribution

Neutrophils, classical monocytes, dendritic cells, mucosal epithelium

Function
Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor
flagellin
Cell Type
Dendritic cells, Epithelial cells, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References
  1. Hayashi F, et al. 2001. Nature. 410:1099
  2. Gewirtz AT, et al. 2001. J Immunol. 167:1882
  3. Smith KD, et al. 2002. Curr Top Microbiol Immunol. 270:93
  4. Bambou JC, et al. 2004. J Biol Chem. 279:42984
  5. Sebastiani G, et al. 2000. Genomics. 64:230
  6. Muzio M, et al. 2000. Biochem Soc Trans. 28:563
Gene ID
7100 View all products for this Gene ID
UniProt
View information about CD285 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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