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Alexa Fluor® 488 anti-human HLA-G Antibody

Pricing & Availability
Clone
87G (See other available formats)
Regulatory Status
RUO
Other Names
Human Leukocyte Antigen-G
Isotype
Mouse IgG2a, κ
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Product Citations
publications
87G_A488_HLA-G_Antibody_012518
Human cytotrophoblastic cells (JEG-3 cell line) were stained with HLA-G (clone 87G) Alexa Fluor® 488 (filled histogram) or mouse IgG2a Alexa Fluor® 488 isotype control (open histogram).
  • 87G_A488_HLA-G_Antibody_012518
    Human cytotrophoblastic cells (JEG-3 cell line) were stained with HLA-G (clone 87G) Alexa Fluor® 488 (filled histogram) or mouse IgG2a Alexa Fluor® 488 isotype control (open histogram).
Compare all formats See Alexa Fluor® 488 spectral data
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335917 25 tests £105
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335918 100 tests £238
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Description

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HLA-G transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.4

Application References

(PubMed link indicates BioLegend citation)
  1. Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
  2. Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
  3. Lila N, et al. 2002. Circulation. 105:1949.
  4. Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
  5. Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed
Product Citations
  1. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
RRID
AB_2728295 (BioLegend Cat. No. 335917)
AB_2728295 (BioLegend Cat. No. 335918)

Antigen Details

Structure
Ig
Distribution

Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors

Function
Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
Ligand/Receptor
ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
Cell Type
Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Hunt JS, et al. 2005. FASEB J. 19:681.
2. Carosella ED, et al. 2008. Blood 111:4862.

Gene ID
3135 View all products for this Gene ID
UniProt
View information about HLA-G on UniProt.org
Go To Top Version: 1    Revision Date: 01/26/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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