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Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
W20119E (See other available formats)
Regulatory Status
RUO
Other Names
EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20119E_AF488_CD326_Antibody_1_102924
TE-71 cells were stained with anti-mouse CD326 (Ep-CAM) (clone W20119E) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
  • W20119E_AF488_CD326_Antibody_1_102924
    TE-71 cells were stained with anti-mouse CD326 (Ep-CAM) (clone W20119E) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
  • W20119E_AF488_CD326_Antibody_2_102924
    IHC staining of Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) (clone W20119E) on C57BL/6 mouse frozen kidney. After fixing with 4% paraformaldehyde (PFA) for 10 minutes, the tissue was blocked and incubated without (panel A) or with (panel B) 10 μg/mL of Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) (clone W20119E) (green). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured with a 10X objective. Scale bar: 200 μm
See Alexa Fluor® 488 spectral data
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112203 25 µg £100
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112204 100 µg £168
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Description

Ep-CAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of Ep-CAM seems to correlate inversely with the level of E-cadherin (CD324). Ep-CAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume.For immunohistochemistry on frozen tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types

Function
Mediates calcium-independent homophilic cell-cell adhesion
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Antigen References
  1. Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
  2. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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